Microsatellite-based paternity analysis in polyploid sweetpotato

被引:23
作者
Buteler, MI
LaBonte, DR [1 ]
Jarret, RL
Macchiavelli, RE
机构
[1] Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Dept Hort, Baton Rouge, LA 70803 USA
[2] USDA, Agr Res Serv, Griffin, GA 30223 USA
[3] Univ Puerto Rico, Dept Agron & Soils, Mayaguez, PR 00681 USA
关键词
molecular markers; genealogy reconstruction; paternity exclusion; most-likely parent; Ipomoea batatas;
D O I
10.21273/JASHS.127.3.392
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Using codominant molecular markers (microsatellites) for paternity identification was investigated in hexaploid sweetpotato Ipomoea batatas (L.) Lam.]. Two experimental populations (CIP and LAES), each consisting or progeny of known parentage, were scored for the presence or absence of alleles segregating at IB-316 and IB-318 microsatellite loci. Paternity was assessed using paternity exclusion and the most-likely parent methods. In the former, paternity is assigned based on the identification of incompatible parent-progeny marker data. In contrast, the latter method incorporates paternity exclusion and a log-likelihood or LOD score that weighs progeny allelic patterns as to the likelihood that they could have come from a given paternal parent. The number of correctly allocated progeny differed for the methods. Paternity exclusion correctly allocated 7% and 25 % of the progeny in the LAES and CIP populations, respectively. The most-likely parent method correctly allocated 23% and 88% of the progeny in the LAES and CIP populations, respectively. The greater misassignments in the LAES population were attributed to low allelic diversity at the LAES IB-318 locus and a larger parental population. This study demonstrates the feasibility of identifying paternity in sweetpotato using a minimal number of loci.
引用
收藏
页码:392 / 396
页数:5
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