Development of a dual fluorogenic and chromogenic dipeptidyl peptidase IV substrate

被引：36
作者：
Ho, NH [1 ]
Weissleder, R [1 ]
Tung, CH [1 ]
机构：
[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Mol Imaging Res, Charlestown, MA 02129 USA
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2006年 / 16卷 / 10期
关键词：
dipeptidyl peptidases DPP-IV; fluorogenic; chromogenic; fluorescent assay;
D O I：
10.1016/j.bmcl.2006.02.045
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A new far-red dual fluorogenic and chromogenic substrate, 5-glycylprolylglycylprolyl-9-di-3-sulfonyl-propylaminobenza[a]phenoxazonium perchlorate (GPGP-2SBPO), wits developed for dipeptidyl peptidase IV (DPP-IV) sensing. The glycylprolylglycylprolyl tetrapeptide was chosen as the recognition sequence due to its stability under physiological conditions. In contrast, the truncated substrate, GP-2SBPO, containing only a glycylprolyl peptide, is unstable. Proteolysis of GPGP-2SBPO was assayed by monitoring the absorbance and fluorescence signals from the released fluorochrome, 2SBPO, at 625 and 670 nm, respectively. (C) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2599 / 2602
页数：4
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