Nuclear overhauser effect investigation on GM1 ganglioside containing N-glycolyl-neuraminic acid (II(3)Neu5GcGgOse(4)Cer)

被引：20

作者：

Brocca, P ^{[1
]}

Acquotti, D ^{[1
]}

Sonnino, S ^{[1
]}

机构：

[1] UNIV MILAN,SCH MED,DEPT MED CHEM & BIOCHEM,STUDY CTR FUNCT BIOCHEM BRAIN LIPIDS,I-20133 MILAN,ITALY

来源：

GLYCOCONJUGATE JOURNAL | 1996年 / 13卷 / 01期

关键词：

gangliosides; N-glycolyl-neuraminic acid; NMR; conformation; nOe;

D O I：

10.1007/BF01049680

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conformational properties of the oligosaccharide chain of GM1 ganglioside containing N-glycolyl-neuraminic acid, beta-Gal-(1-3)-beta-GalNAc-(1-4)-[alpha-Neu5Gc-(2-3)]-beta-Gal-(1-4)-beta-Glc-(1-1)-Cer, were studied through NMR nuclear Overhauser effect investigations on the monomeric ganglioside in dimethylsulfoxide, and on mixed micelles of ganglioside and dodecylphosphocholine in water. Several interresidual contacts for the trisaccharide core -beta-GalNAc-(1-4)-[alpha-Neu5Gc-(2-3)]-beta-Gal- were found to fix the relative orientation of the three saccharides, while the glycosidic linkage of the terminal beta-Gal- was found to be quite mobile as the beta-Gal-(1-3)-beta-GalNAc- disaccharide exists in different conformations. These results are similar to those found for two GM1 gangliosides containing N-acetyl-neuraminic acid and neuraminic acid [1].

引用

页码：57 / 62

页数：6

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