Development of SSR markers and association studies of markers with phenology and yield-related traits in grass pea (Lathyrus sativus)

被引:7
作者
Soren, Khela Ram [1 ]
Konda, Aravind Kumar [1 ]
Gangwar, Priyanka [2 ]
Tiwari, Vijay A. [3 ]
Shanmugavadivel, P. S. [1 ]
Parihar, Ashok Kumar [1 ]
Dixit, Girish Prasad [1 ]
Singh, Narendra Pratap [1 ]
机构
[1] ICAR Indian Inst Pulses Res, Kanpur 208024, Uttar Pradesh, India
[2] Amity Univ, Amity Inst Biotechnol, Lucknow 226028, Uttar Pradesh, India
[3] Chatrapati Shahu Ji Maharajah Univ, Dept Biotechnol, Kanpur 208024, Uttar Pradesh, India
关键词
gene flow; genetic improvements; PIC; population structure; putative functions; POPULATION-STRUCTURE; SOFTWARE; SEQUENCE; PROVIDES; COMPLEX;
D O I
10.1071/CP19557
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Grass pea (Lathyrus sativusL.) is an important food crop cultivated in dryland agricultural ecosystem. It is an important source of dietary protein to millions of people living in low-income countries in South-east Asia and Africa. The present study emphasises the development of genomic resources and their application in marker-trait association for plant phenology and yield-related traits in lathyrus.In silicomining of nucleotide sequences identified 203 simple sequence repeat (SSR) motifs, of which trimer repeats (62%) were most abundant followed by tetramer (19%), hexamer (10%), pentamer (6%) and dimer (3%) nucleotide repeats. Of 150 SSR markers screened, 60 markers were amplified 75 alleles from 50 germplasm lines with 2-3 alleles per locus and the polymorphic information content of 0.45 was observed. We report 6 significant marker-trait associations using the developed SSR markers for plant phenology and yield-related traits following mixed linear model (Q+K) analysis. Gene ontology search of trait linked markers revealed marker regions encoding genes related to homeobox-leucine zipper protein ATHB-6-like, rubredoxin family protein, and cationic peroxidise. Understanding the association of novel alleles in trait expression will play a significant role in future lathyrus crop improvement programmes.
引用
收藏
页码:768 / 775
页数:8
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