Aptamer-based impedimetric determination of the human blood clotting factor IX in serum using an interdigitated electrode modified with a ZnO nanolayer

被引：27

作者：

Cheen, Ong Chong ^{[1
,2
]}

Gopinath, Subash C. B. ^{[1
,3
]}

Perumal, Veeradasan ^{[1
,4
]}

Arshad, M. K. Md ^{[1
]}

Lakshmipriya, Thangavel ^{[1
]}

Chen, Yeng ^{[5
,6
]}

Haarindraprasad, R. ^{[1
]}

Rao, Balakrishnan S. ^{[1
,7
]}

Hashim, Uda ^{[1
]}

Pandian, Kannaiyan ^{[8
]}

机构：

[1] Univ Perlis, INEE, Kangar 01000, Perlis, Malaysia

[2] Univ Malaysia Perlis, Fac Engn Technol, Kangar 01000, Perlis, Malaysia

[3] Univ Malaysia Perlis, Sch Bioproc Engn, Arau 02600, Perlis, Malaysia

[4] Univ Teknol PETRONAS, Ctr Innovat Nanostruct & Nanodevices COINN, Bandar Seri Iskandar 32610, Perak Darul Rid, Malaysia

[5] Dept Oral Biol & Craniofacial Sci, Kuala Lumpur, Malaysia

[6] Univ Malaya, Fac Dent, OCRCC, Kuala Lumpur 50603, Malaysia

[7] USIM, Fak Kejuruteraan & Alam Bina, Nilai 71800, Negeri Sembilan, Malaysia

[8] Univ Madras, Dept Inorgan Chem, Guindy Campus, Madras 600025, Tamil Nadu, India

来源：

MICROCHIMICA ACTA | 2017年 / 184卷 / 01期

关键词：

Coagulation; Hemophilia; Impedance; Resistance analysis; Factor IX; Aptamer; Monoclonal antibody; Clotting; RNA APTAMER; GOLD NANOPARTICLES; SENSORS; BINDING; ASSAY; COAGULATION; SENSITIVITY; BIOSENSOR; RESONANCE; BLOCKING;

D O I：

10.1007/s00604-016-2001-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article describes a sensitive impedimetric method for the determination of human blood coagulation factor IX protein (FIX) which is present in extremely low concentration in serum. An interdigitated electrode (IDE) whose surface was layered with zinc oxide was modified with two kinds of probes. One is an antibody, the other an aptamer against FIX. A comparative study between anti-FIX aptamer and anti-FIX antibody showed the aptamer to possess higher affinity for FIX. A sandwich aptamer assay was worked out by using the FIX-binding aptamer on the surface of the IDE. It has a detection limit as low as 10 pM which makes it 4 to 30-fold more sensitive than any other method reported for FIX. Moreover, to practice detection in clinical samples, FIX was detected from the human blood serum by spiking. In our perception, the sensitivity of the ZnO-modified IDE presented here makes it a promising tool for sensing clinically relevant analytes that are present in very low (sub-pM) concentrations.

引用

页码：117 / 125

页数：9

共 35 条

[1] A Point-of-Care Immunosensor for Human Chorionic Gonadotropin in Clinical Urine Samples Using a Cuneated Polysilicon Nanogap Lab-on-Chip [J].

Balakrishnan, S. R. ;

Hashim, U. ;

Gopinath, Subash C. B. ;

Poopalan, P. ;

Ramayya, H. R. ;

Omar, M. Iqbal ;

Haarindraprasad, R. ;

Veeradasan, P. .

PLOS ONE, 2015, 10 (09)

[2]

Busher J.T., 1990, Clinical Methods: The History, Physical, and Laboratory Examinations, V3rd ed.

[3] Detection of colored nanomaterials using evanescent field-based waveguide sensors [J].

Fujimaki, Makoto ;

Nomura, Ken-ichi ;

Sato, Kazuki ;

Kato, Takafumi ;

Gopinath, Subash C. B. ;

Wang, Xiaomin ;

Awazu, Koichi ;

Ohki, Yoshimichi .

OPTICS EXPRESS, 2010, 18 (15) :15732-15740

[4]

Gerwen P., 1997, PROC INT SOLID STATE, V2, P73, DOI [DOI 10.1109/SENSOR.1997.635249, 10.1109/SENSOR.1997.635249]

[5]

Gopinath S. C. B., 2016, MICROCHIM ACTA

[6] Influence of nanometric holes on the sensitivity of a waveguide-mode sensor: Label-free nanosensor for the analysis of RNA aptamer-ligand interactions [J].

Gopinath, Subash C. B. ;

Awazu, Koichi ;

Fujimaki, Makoto ;

Sugimoto, Katsuaki ;

Ohki, Yoshimichi ;

Komatsubara, Tetsuro ;

Tominaga, Junji ;

Gupta, Kailash C. ;

Kumar, Penmetcha K. R. .

ANALYTICAL CHEMISTRY, 2008, 80 (17) :6602-6609

[7] Snake-venom-derived factor IX-binding protein specifically blocks the γ-carboxyglutamic acid-rich-domain-mediated membrane binding of human factors IX and X [J].

Gopinath, Subash C. B. ;

Shikamoto, Yasuo ;

Mizuno, Hiroshi ;

Kumar, Pennnetcha K. R. .

BIOCHEMICAL JOURNAL, 2007, 405 (351-357) :351-357

[8] A potent anti-coagulant RNA aptamer inhibits blood coagulation by specifically blocking the extrinsic clotting pathway [J].

Gopinath, Subash C. B. ;

Shikamoto, Yasuo ;

Mizuno, Hiroshi ;

Kumar, Penmetcha K. R. .

THROMBOSIS AND HAEMOSTASIS, 2006, 95 (05) :767-771

[9] An RNA aptamer that discriminates bovine factor IX from human factor IX [J].

Gopinath, Subash C. B. ;

Balasundaresan, Dhakshnamoorthy ;

Akitomi, Joe ;

Mizuno, Hiroshi .

JOURNAL OF BIOCHEMISTRY, 2006, 140 (05) :667-676

[10] Current aspects in immunosensors [J].

Gopinath, Subash C. B. ;

Tang, Thean-Hock ;

Citartan, Marimuthu ;

Chen, Yeng ;

Lakshmipriya, Thangavel .

BIOSENSORS & BIOELECTRONICS, 2014, 57 :292-302

← 1 2 3 4 →