Identification of Gammaretroviruses Constitutively Released from Cell Lines Used for Human Immunodeficiency Virus Research

被引：220

作者：

Takeuchi, Yasuhiro ^{[2
]}

McClure, Myra O. ^{[1
]}

Pizzato, Massimo ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Div Med, Dept Infect Dis, London W2 1PG, England

[2] UCL, Wohl Vir Ctr, Div Infect & Immun, London W1T 4JF, England

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 24期

关键词：

D O I：

10.1128/JVI.01726-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Three human cell lines used in human immunodeficiency virus research were found to be contaminated with previously undetected retroviruses. On the bases of partial nucleotide sequence, capsid protein antigenicity, vector mobilization, and receptor usage studies, these contaminants were shown to be replication competent and to belong to the Gammaretrovirus genus. While the TZM-bl cells harbor ecotropic murine leukemia virus (MLV), Jurkat J6 cells were found to release xenotropic MLV and the A3.01/F7 cells to produce gibbon ape leukemia virus. These findings highlight the importance of routine testing of cell lines for retrovirus contamination to prevent potential experimental artifacts and allow correct biohazard assessment.

引用

页码：12585 / 12588

页数：4

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