Bioelectronic nose for methyl mercaptan vapor using a xenobiotic metabolizing system

被引：0

作者：

Mitsubayashi, K ^{[1
]}

Hashimoto, Y ^{[1
]}

机构：

[1] Tokai Univ, Dept Elect Engn, Kanagawa 2591292, Japan

来源：

TRANSDUCERS '01: EUROSENSORS XV, DIGEST OF TECHNICAL PAPERS, VOLS 1 AND 2 | 2001年

关键词：

bioelectronic nose; methyl mercaptan; xenobiotic metabolism;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A bioelectronic nose (Bio-nose) for methyl mercaptan (MM) in the gas-phase was constructed a flavin-containing monooxygenase 3 (FMO3) and a reaction cell with both gas- and liquid-compartments separated by a porous hydrophobic membrane. The FMO immobilized electrode was calibrated against MM solutions from 0.03 to 4.98 mmol/l since the characteristics of the FMO3 immobilized electrode was improved by applying a substrate regeneration cycle with ascorbic acid as reducing reagent, The bio-nose with the FMO3 immobilized membrane could be used to measure MIA in the gas phase, and possessed high selectivity being attribute to the FMO3 substrate specificity, continuous measurability and good reproducibility.

引用

页码：346 / 349

页数：4

共 8 条

[1] Missense mutation in flavin-containing mono-oxygenase 3 gene, FMO3, underlies fish-odour syndrome [J].

Dolphin, CT ;

Janmohamed, A ;

Smith, RL ;

Shephard, EA ;

Phillips, IR .

NATURE GENETICS, 1997, 17 (04) :491-494

[2]

Hasebe Y., 1998, CHEM SENSORS, V14, P116

[3] A CONVENIENT PHOTOCHEMICAL METHOD TO IMMOBILIZE ENZYMES [J].

ICHIMURA, K .

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 1984, 22 (11) :2817-2828

[4] Isoform specificity of trimethylamine N-oxygenation by human flavin containing monooxygenase (FMO) and P450 enzymes -: Selective catalysis by FMO3 [J].

Lang, DH ;

Yeung, CK ;

Peter, RM ;

Ibarra, C ;

Gasser, R ;

Itagaki, K ;

Philpot, RM ;

Rettie, AE .

BIOCHEMICAL PHARMACOLOGY, 1998, 56 (08) :1005-1012

[5] Development of a gas-phase biosensor for trimethylamine using a flavin-containing monooxygenase 3 [J].

Mitsubayashi, K ;

Hashimoto, Y .

ELECTROCHEMISTRY, 2000, 68 (11) :901-903

[6] ANALYSIS OF METABOLITES IN SWEAT AS A MEASURE OF PHYSICAL CONDITION [J].

MITSUBAYASHI, K ;

SUZUKI, M ;

TAMIYA, E ;

KARUBE, I .

ANALYTICA CHIMICA ACTA, 1994, 289 (01) :27-34

[7] METHANE AND BUTANE CONCENTRATIONS IN A MIXTURE WITH AIR DETERMINED BY MICROCALORIMETRIC SENSORS AND NEURAL NETWORKS [J].

SOMMER, V ;

TOBIAS, P ;

KOHL, D .

SENSORS AND ACTUATORS B-CHEMICAL, 1993, 12 (02) :147-152

[8]

1998, INT CHEM SAFETY CARD

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