On-chip detection of multiple serum antibodies against epitopes of celiac disease by an array of amorphous silicon sensors

被引:29
作者
Costantini, Francesca [1 ]
Nascetti, Augusto [2 ]
Scipinotti, Riccardo [3 ]
Domenici, Fabio [4 ]
Sennato, Simona [5 ]
Gazza, Laura [5 ]
Bordi, Federico [4 ]
Pogna, Norberto [5 ]
Manetti, Cesare [1 ]
Caputo, Domenico [3 ]
de Cesare, Giampiero [3 ]
机构
[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy
[2] Univ Roma La Sapienza, Dipartimento Ingn Astronaut Elettr & Energet, I-00138 Rome, Italy
[3] Univ Roma La Sapienza, Dipartimento Ingn Informaz Elettron & Telecomunic, I-00184 Rome, Italy
[4] Univ Roma La Sapienza, Dipartimento Fis, I-00185 Rome, Italy
[5] Consiglio Ric & Sperimentaz Agr CRA QCE, I-00191 Rome, Italy
来源
RSC ADVANCES | 2014年 / 4卷 / 04期
关键词
MICROARRAY TECHNOLOGY; PROTEIN MICROARRAYS; POLYMER BRUSHES; ELECTROCHEMICAL IMMUNOSENSOR; ULTRAVIOLET SENSOR; DIAGNOSIS; FILM; IMMOBILIZATION; OCHRATOXIN; BIOSENSOR;
D O I
10.1039/c3ra46058d
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In this paper, we present the preliminary results of an ELISA-on-chip device, intended as a technological demonstrator of a novel analytical system suitable for the diagnosis and follow-up of celiac disease. The idea of the work is to combine an array of amorphous silicon photosensors with a pattern of a poly(2-hydroxyethyl methacrylate) polymer brush film, which acts as anchor for the immobilization of gliadin peptides containing the celiac disease epitopes. Recognition relies on a sandwich immunoassay between antibodies against the peptides and secondary antibodies marked with horseradish peroxidase to obtain a chemiluminescent signal. Detection is based on the measurement of photocurrent induced in the array of amorphous silicon photosensors by the chemiluminescent signal. An ad-hoc procedure has been developed in order to enable the fabrication of the photodiode array and the polymer brush pattern on the two sides of the same glass substrate ensuring the compatibility of the different technological steps. The sensitivity and the selectivity of the chip for multiplex immunoassays were demonstrated using two gliadin peptides (VEA and DEC). In particular, we found that the average amount of the bound HRP revealed by our analytical protocol is 3.5(+/- 0.3) x 10(-6) pg mu m(-2) and 0.85(+/- 0.3) x 10(-6) pg mu m(-2) for specific and non-specific interactions, respectively.
引用
收藏
页码:2073 / 2080
页数:8
相关论文
共 63 条
[61]   Diagnostic devices as biomaterials: a review of nucleic acid and protein microarray surface performance issues [J].
Wu, Peng ;
Castner, David G. ;
Grainger, David W. .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2008, 19 (06) :725-753
[62]   Point-of-care diagnostics for global health [J].
Yager, Paul ;
Domingo, Gonzalo J. ;
Gerdes, John .
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2008, 10 :107-144
[63]   Protein Microarrays for Personalized Medicine [J].
Yu, Xiaobo ;
Schneiderhan-Marra, Nicole ;
Joos, Thomas O. .
CLINICAL CHEMISTRY, 2010, 56 (03) :376-387