Structural characteristics of water-extractable nonstarch polysaccharides from barley malt

被引:18
作者
Cyran, M
Izydorczyk, MS
MacGregor, AW
机构
[1] Inst Plant Breeding, Dept Nutr Evaluat Plant Mat, PL-05870 Blonie, Poland
[2] Grain Res Lab, Winnipeg, MB R3C 3G8, Canada
关键词
D O I
10.1094/CCHEM.2002.79.3.359
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Water-extractable (WE) material was isolated from a Canadian barley malt (cv. Harrington). The purified WE material contained mainly arabinoxylans, beta-glucans, proteins, and small amounts of arabinogalactans and mannose-containing polymers. WE material was treated with specific enzymes to obtain two fractions: one enriched in arabinoxylan (AX) and another enriched in beta-glucan (BG). The AX fraction was further fractionated by stepwise precipitation in (NH4)(2)SO4 into five arabinoxylan subfractions. H-1-NMR spectroscopy and sugar analyses revealed a relatively high content of unsubstituted xylose residues (48-58%) as well as a relatively high content of doubly substituted xylose residues (28-33%) in the structure of the arabinoxylans. beta-Glucans constituted a minor portion of water-extractable malt polysaccharides and were characterized by high levels of tri- and tetrasaccharide residues (93.4%) with a molar ratio of 2.19 for cellotriosyl to cellotetraosyl units. Size-exclusion chromatography revealed that the WE material contained several polymer populations. One population had a very high molecular weight that appeared to be the result of aggregation. The AX fraction contained higher molecular weight polymers than the BG fraction.
引用
收藏
页码:359 / 366
页数:8
相关论文
共 56 条
[41]   Effects of cultivar and environment on beta-(1,3)-(1,4)-D-glucan content and acid extract viscosity of Spanish barleys [J].
PerezVendrell, AM ;
Brufau, J ;
MolinaCano, JL ;
Francesch, M ;
Guasch, J .
JOURNAL OF CEREAL SCIENCE, 1996, 23 (03) :285-292
[42]   Variation in the degree of D-xylose substitution in water-extractable European durum wheat (Triticum durum Desf.) semolina arabinoxylans [J].
Roels, SP ;
Collado, M ;
Loosveld, AM ;
Grobet, PJ ;
Delcour, JA .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (05) :1813-1816
[43]   NEW INVESTIGATIONS OF THE STRUCTURE OF GRAPE ARABINOGALACTAN PROTEIN [J].
SAULNIER, L ;
BRILLOUET, JM ;
MOUTOUNET, M ;
DUPENHOAT, CH ;
MICHON, V .
CARBOHYDRATE RESEARCH, 1992, 224 :219-235
[44]  
SCHWARZ PB, 1995, J AM SOC BREW CHEM, V53, P157
[45]   OCCURRENCE AND NATURE OF FERULIC ACID SUBSTITUTION OF CELL-WALL POLYSACCHARIDES IN GRAMINACEOUS PLANTS [J].
SMITH, MM ;
HARTLEY, RD .
CARBOHYDRATE RESEARCH, 1983, 118 (JUL) :65-80
[46]   STRUCTURAL FEATURES OF ARABINOXYLANS FROM BARLEY AND MALT CELL-WALL MATERIAL [J].
VIETOR, RJ ;
ANGELINO, SAGF ;
VORAGEN, AGJ .
JOURNAL OF CEREAL SCIENCE, 1992, 15 (03) :213-222
[47]   STRUCTURES OF SMALL OLIGOMERS LIBERATED FROM BARLEY ARABINOXYLANS BY ENDOXYLANASE FROM ASPERGILLUS-AWAMORI [J].
VIETOR, RJ ;
HOFFMANN, RA ;
ANGELINO, SAGF ;
VORAGEN, AGJ ;
KAMERLING, JP ;
VLIEGENTHART, JFG .
CARBOHYDRATE RESEARCH, 1994, 254 :245-255
[48]   NONSTARCH POLYSACCHARIDES IN BARLEY AND MALT - A MASS BALANCE OF FLOUR FRACTIONATION [J].
VIETOR, RJ ;
VORAGEN, AGJ ;
ANGELINO, SAGF ;
PILNIK, W .
JOURNAL OF CEREAL SCIENCE, 1991, 14 (01) :73-83
[49]  
VINKX CJA, 1993, CEREAL CHEM, V70, P311
[50]  
WAINWRIGHT T, 1990, BREWERS GUARDIAN, V119, P9