Sulfur-oxidizing bacteria dominate the microbial diversity shift during the pyrite and low-grade pyrolusite bioleaching process

被引：21

作者：

Han, Yifan ^{[1
]}

Ma, Xiaomei ^{[1
]}

Zhao, Wei ^{[1
]}

Chang, Yunkang ^{[1
]}

Zhang, Xiaoxia ^{[1
]}

Wang, Xingbiao ^{[1
]}

Wang, Jingjing ^{[1
]}

Huang, Zhiyong ^{[1
]}

机构：

[1] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Eng, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2013年 / 116卷 / 04期

基金：

国家高技术研究发展计划(863计划);

关键词：

16S rRNA gene clone library; Bioleaching; Pyrite-pyrolusite; Sulfur-oxidizing bacteria; THIOMONAS-ARSENIVORANS; SIMPSON DIVERSITY; SULFIDE ORE; MANGANESE; STRAIN; MECHANISM; COMMUNITY; OXIDATION; DYNAMICS; REACTORS;

D O I：

10.1016/j.jbiosc.2013.04.012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The microbial ecology of the pyrite-pyrolusite bioleaching system and its interaction with ore has not been well-described. A 16S rRNA gene clone library was created to evaluate changes in the microbial community at different stages of the pyrite-pyrolusite bioleaching process in a shaken flask. The results revealed that the bacterial community was disturbed after 5 days of the reaction. Phylogenetic analysis of 16S rRNA sequences demonstrated that the predominant microorganisms were members of a genus of sulfur-oxidizing bacteria, Thiomonas sp., that subsequently remained dominant during the bioleaching process. Compared with iron-oxidizing bacteria, sulfur-oxidizing bacteria were more favorable to the pyrite-pyrolusite bioleaching system. Decreased pH due to microbial acid production was an important condition for bioleaching efficiency. Iron-oxidizing bacteria competed for pyrite reduction power with Mn(IV) in pyrolusite under specific conditions. These results extend our knowledge of microbial dynamics during pyrite-pyrolusite bioleaching, which is a key issue to improve commercial applications. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：465 / 471

页数：7

共 32 条

[1] Studies on reaction mechanism of bioleaching of manganese ore [J].