A study on anti-oxidative activity of soybean peptides with linoleic acid peroxidation systems

被引：4
|
作者：
Xu, L
Li, HM
Huang, YB
Wang, H
Nie, GJ
Zhang, XZ [1 ]
机构：
[1] Jilin Univ, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130021, Peoples R China
[2] Jilin Agr Univ, Coll Food Engn, Changchun 130018, Peoples R China
[3] Jilin Univ, China Japan Union Hosp, Changchun 130033, Peoples R China
来源：
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES | 2006年 / 22卷 / 02期
关键词：
soybean bioactive peptides; anti-oxidative activity; linoleic acid peroxidation system;
D O I：
10.1016/S1005-9040(06)60077-9
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Soybean bioactive peptides (SBPs) were prepared from the isolated soybean protein by proteolysis with an alkaline protease, alcalase, at 50 degrees C and pH = 8.0. The dependence of hydrolysis time on hydrolysis degree and molecular weight distribution were examined. The hydrolysate was fractionated on a Sephadex G-25 column and the anti-oxidative activities of the fractions were detected by the method of pyrogallol auto-oxidation. The average chain length of soybean peptides that have anti-oxidative activity was estimated to be about 7. The anti-oxidative properties of the soybean peptide were also studied by using linoleic acid peroxidation systems. The optimal condition of the peroxidation system was set up, Vc/Cu2+ as the inducer at pH = 7. 4 and 25 degrees C. In addition, soybean peptides show higher antioxidative activity compared with GSH.
引用
收藏
页码：205 / 208
页数：4
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