A cost effective real-time PCR for the detection of adenovirus from viral swabs

被引：10

作者：

Al-Siyabi, Turkiya ^{[1
]}

Binkhamis, Khalifa ^{[1
]}

Wilcox, Melanie ^{[2
]}

Wong, Sallene ^{[3
]}

Pabbaraju, Kanti ^{[3
]}

Tellier, Raymond ^{[3
,4
]}

Hatchette, Todd F. ^{[1
,2
]}

LeBlanc, Jason J. ^{[1
,2
]}

机构：

[1] Capital Dist Hlth Author, Dept Pathol & Lab Med, Div Microbiol, Halifax, NS B3H 1V8, Canada

[2] Dalhousie Univ, Halifax, NS, Canada

[3] Prov Lab Publ Hlth, Calgary, AB, Canada

[4] Univ Calgary, Calgary, AB, Canada

来源：

VIROLOGY JOURNAL | 2013年 / 10卷

关键词：

Homogenization; Extraction; Real-time PCR; Adenovirus; Cost analysis; RESPIRATORY-TRACT INFECTIONS; NUCLEIC-ACID EXTRACTION; QUANTITATIVE DETECTION; MULTIPLEX PCR; ASSAY; PANEL; DNA; CANADA; QUANTIFICATION; PNEUMONIA;

D O I：

10.1186/1743-422X-10-184

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories. To overcome fiscal challenges, a cost effective strategy was developed using a combination of homogenization and heat treatment with an "in-house" real-time PCR. In 196 swabs submitted for adenovirus detection, this crude extraction method showed performance characteristics equivalent to viral DNA obtained from a commercial nucleic acid extraction. In addition, the in-house real-time PCR outperformed traditional testing strategies using virus culture, with sensitivities of 100% and 69.2%, respectively. Overall, the combination of homogenization and heat treatment with a sensitive in-house real-time PCR provides accurate results at a cost comparable to viral culture.

引用

页数：12

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