Determination of theoretical calculations by DFT method and investigation of antioxidant, antimicrobial properties of olive leaf extracts from different regions

In this study, we studied samples of mature olive leaves from the districts of Incirli Ova within the province of Aydin and the district of Fethiye in Mugla/Turkey. Several processes were carried out on the olive leaves to use them in this study, including drying under different conditions, determination of moisture, extract output, overall determination of phenols, antioxidant activity determination and anti-microbial assays. The chemicals that were used in the study were Folin's reagent and gallic acid for total phenolic assays, DPPH (1,1-diphenyl-2-picrylhydrazyl) and Trolox for antioxidant activity assays and nutrient broth and nutrient agar for antimicrobial testing. In the theoretical part of the study, the structures of oleuropein and Trolox molecules were examined, and their oxidation properties were aimed to be determined and compared to experimental results. According to the results of total phenolic assays, the phenol contents in the olive leaves from Aydin and Mugla were observed to be very close to each other. In the anti-microbial assay, it was observed that the samples of olive leaves from Mugla were more antioxidant than those from Aydin.