Preparation of Cationic Nanogels for Nucleic Acid Delivery

被引：66
作者：
Averick, Saadyah E. [1 ]
Paredes, Eduardo [2 ]
Irastorza, Ainara [3 ]
Shrivats, Arun R. [3 ]
Srinivasan, Abiraman [1 ]
Siegwart, Daniel J. [1 ]
Magenau, Andrew J. [1 ]
Cho, Hong Y. [1 ]
Hsu, Eric [3 ]
Averick, Amram A. [1 ]
Kim, Jinku [3 ]
Liu, Shiguang [3 ]
Hollinger, Jeffrey O. [3 ]
Das, Subha R. [2 ]
Matyjaszewski, Krzysztof [1 ]
机构：
[1] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA
[2] Carnegie Mellon Univ, Ctr Nucle Acids Sci & Technol, Pittsburgh, PA 15213 USA
[3] Carnegie Mellon Univ, Dept Biomed Engn & Biol Sci, Bone Tissue Engn Ctr, Pittsburgh, PA 15219 USA
来源：
BIOMACROMOLECULES | 2012年 / 13卷 / 11期
基金：
美国国家科学基金会;
关键词：
TRANSFER RADICAL POLYMERIZATION; GENE DELIVERY; SIRNA DELIVERY; STAR POLYMERS; PROTEIN; DRUG; ATRP; RNA; DNA; NANOPARTICLES;
D O I：
10.1021/bm301166s
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cationic nanogels with, site-selected functionality were designed for the delivery of nucleic acid payloads targeting numerous therapeutic applications. Functional cationic nanogels containing quaternized 2,(dimethylamino)-ethyl methacrylate and a cross linker with reducible disulfide moieties.(qNG) were prepared by activators generated by electron transfer (AGET) atom transfer radical polymerization (ATRP) in an inverse miniemulsion. Polyplex formation between the qNG and nucleic acid exemplified by plasmid. DNA (pDNA) and short interfering RNA (siRNA duplexes) were evaluated The,delivery of polyplexes was optimized for the delivery of pDNA and siRNA to the Drosophila Schneider 2 (S2) cell-line. The qNG/nucleic acid (i.e., siRNA and pDNA) polyplexes were found to be highly effective in their capabilities to deliver their respective payloads.
引用
收藏
页码：3445 / 3449
页数：5
相关论文
共 53 条
  • [1] RNAi therapeutics: Principles, prospects and challenges
    Aagaard, Lars
    Rossi, John J.
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 2007, 59 (2-3) : 75 - 86
  • [2] Anderson ME, 1998, CHEM-BIOL INTERACT, V112, P1
  • [3] Association and dissociation characteristics of polymer/DNA complexes used for gene delivery
    Arigita, C
    Zuidam, NJ
    Crommelin, DJA
    Hennink, WE
    [J]. PHARMACEUTICAL RESEARCH, 1999, 16 (10) : 1534 - 1541
  • [4] ATRP under Biologically Relevant Conditions: Grafting from a Protein
    Averick, Saadyah
    Simakova, Antonina
    Park, Sangwoo
    Konkolewicz, Dominik
    Magenau, Andrew J. D.
    Mehl, Ryan A.
    Matyjaszewski, Krzysztof
    [J]. ACS MACRO LETTERS, 2012, 1 (01): : 6 - 10
  • [5] Direct DNA Conjugation to Star Polymers for Controlled Reversible Assemblies
    Averick, Saadyah
    Paredes, Eduardo
    Li, Wenwen
    Matyjaszewski, Krzysztof
    Das, Subha R.
    [J]. BIOCONJUGATE CHEMISTRY, 2011, 22 (10) : 2030 - 2037
  • [6] A Protein-Polymer Hybrid Mediated By DNA
    Averick, Saadyah E.
    Paredes, Eduardo
    Grahacharya, Debasish
    Woodman, Bradley F.
    Miyake-Stoner, Shigeki J.
    Mehl, Ryan A.
    Matyjaszewski, Krzysztof
    Das, Subha R.
    [J]. LANGMUIR, 2012, 28 (04) : 1954 - 1958
  • [7] Covalently incorporated protein-nanogels using AGET ATRP in an inverse miniemulsion
    Averick, Saadyah E.
    Magenau, Andrew J. D.
    Simakova, Antonina
    Woodman, Bradley F.
    Seong, Andrew
    Mehl, Ryan A.
    Matyjaszewski, Krzysztof
    [J]. POLYMER CHEMISTRY, 2011, 2 (07) : 1476 - 1478
  • [8] Anti-fouling magnetic nanoparticles for siRNA delivery
    Boyer, Cyrille
    Priyanto, Priyanto
    Davis, Thomas P.
    Pissuwan, Dakrong
    Bulmus, Volga
    Kavallaris, Maria
    Teoh, Wey Yang
    Amal, Rose
    Carroll, Matt
    Woodward, Robert
    St Pierre, Tim
    [J]. JOURNAL OF MATERIALS CHEMISTRY, 2010, 20 (02) : 255 - 265
  • [9] Synthesis of Biocompatible PEG-Based Star Polymers with Cationic and Degradable Core for siRNA Delivery
    Cho, Hong Y.
    Srinivasan, Abiraman
    Hong, Joanna
    Hsu, Eric
    Liu, Shiguang
    Shrivats, Arun
    Kwak, Dan
    Bohaty, Andrew K.
    Paik, Hyun-jong
    Hollinger, Jeffrey O.
    Matyjaszewski, Krzysztof
    [J]. BIOMACROMOLECULES, 2011, 12 (10) : 3478 - 3486
  • [10] Rapid Cellular Internalization of Multifunctional Star Polymers Prepared by Atom Transfer Radical Polymerization
    Cho, Hong Y.
    Gao, Haifeng
    Srinivasan, Abiraman
    Hong, Joanna
    Bencherif, Sidi A.
    Siegwart, Daniel J.
    Paik, Hyun-jong
    Hollinger, Jeffrey O.
    Matyjaszewski, Krzysztof
    [J]. BIOMACROMOLECULES, 2010, 11 (09) : 2199 - 2203
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