Titanium-containing magnetic mesoporous silica spheres: Effective enrichment of peptides and simultaneous separation of nonphosphopeptides and phosphopeptides

被引:17
作者
Li, Xiao-Shui [1 ]
Su, Xin [1 ]
Zhu, Gang-tian [1 ]
Zhao, Yong [1 ,2 ,3 ]
Yuan, Bi-Feng [1 ]
Guo, Lin [1 ,2 ,3 ]
Feng, Yu-Qi [1 ]
机构
[1] Wuhan Univ, Dept Chem, Key Lab Analyt Chem Biol & Med, Minist Educ, Wuhan 430072, Peoples R China
[2] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
[3] Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China
关键词
Human serum; Magnetic mesoporous silica spheres; Peptides and phosphopeptides; Titanium; SELECTIVE ENRICHMENT; MASS-SPECTROMETRY; ENDOGENOUS PEPTIDES; PEPTIDOMICS; NANOPARTICLES; MICROSPHERES; SERUM; MS; PHOSPHORYLATION; IDENTIFICATION;
D O I
10.1002/jssc.201101067
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Protein phosphorylation is a common posttranslational modification, and involved in many cellular processes. Like endogenous peptides, endogenous phosphopeptides contain many biomarkers of preclinical screening and disease diagnosis. In this work, titanium-containing magnetic mesoporous silica spheres were synthesized and applied for effective enrichment of peptides from both tryptic digests of standard proteins and human serum. Besides, the enriched peptides can be further separated into nonphosphopeptides and phosphopeptides by a simple elution. First, titanium-containing magnetic mesoporous silica spheres were synthesized by a sol-gel method and found to have high surface area, narrow pore size distribution, and useful magnetic responsivity. Then, as the prepared material was used for selective capturing of phosphopeptides, it demonstrated to have higher selectivity than commercial titanium dioxide. Moreover, via combination of size-exclusion mechanism, hydrophobic interaction, and affinity chromatography, titanium-containing magnetic mesoporous silica spheres were successfully applied to simultaneously extract and separate nonphosphopeptides and phosphopeptides from standard protein digestion and human serum.
引用
收藏
页码:1506 / 1513
页数:8
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