APPLICATION OF IMMUNOMAGNETIC PARTICLES TO ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR IMPROVEMENT OF DETECTION SENSITIVITY OF HCG

被引：7

作者：

Kuo, Hsiao-Ting ^{[1
]}

Yeh, Jay Z. ^{[2
]}

Wu, Po-Hua ^{[3
]}

Jiang, Chii-Ming ^{[4
]}

Wu, Ming-Chang ^{[1
]}

机构：

[1] Natl Pingtung Univ Sci & Technol, Dept Food Sci, Nei Pu Hsiang 91201, Pingtung, Taiwan

[2] Northwestern Univ, Feinberg Sch Med, Evanston, IL 60208 USA

[3] Natl Chiayi Univ, Dept Microbiol Immunol & Biophamarceut, Chiayi, Taiwan

[4] Natl Taitung Coll, Food Proc Dept, Taitung, Taiwan

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2012年 / 33卷 / 04期

关键词：

antibody immunomagnetic particle; enzyme-linked immunoassay; human chorionic gonadotropin; magnetic carrier concentration technology; pregnancy test; superparamagnetic particle; HUMAN SERUM; BETA-HCG; IMMUNOASSAY; URINE; PURIFICATION; ALPHA;

D O I：

10.1080/15321819.2012.655820

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This investigation was aimed at using superparamagnetic particles to enzyme-linked immunosorbent assay (SPIO-ELISA) of human chorionic gonadotropin (hCG) to enhance detection sensitivity of hCG. We found that N-(3-dimethyl aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) was the best cross-linking reagent to link anti hCG alpha antibody to superparamagnetic particle (SPIO-anti hCG alpha antibody immunomagnetic particle). To improve the specificity of the assay, a horse radish peroxidase (HRP)-labeled anti-hCG beta monoclonal antibody was used to detect captured hCG using double antibody sandwich ELISA assay. SPIO-ELISA application to determine hCG increased the sensitivity to 1 mIU/mL, which is a level of sensitivity enabling the diagnosis of pregnancy during the early gestational period.

引用

页码：377 / 387

页数：11

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