Infection of primary cultures of human neural cells by human coronaviruses 229E and OC43

被引：69

作者：

Bonavia, A

Arbour, N

Yong, VW

Talbot, PJ

机构：

[1] UNIV QUEBEC,INST ARMAND FRAPPIER,LAB NEUROIMMUNOVIROL,VIROL RES CTR,LAVAL,PQ H7N 4Z3,CANADA

[2] MCGILL UNIV,MONTREAL NEUROL INST,NEUROIMMUNOL UNIT,MONTREAL,PQ H3A 2B4,CANADA

来源：

JOURNAL OF VIROLOGY | 1997年 / 71卷 / 01期

关键词：

D O I：

10.1128/JVI.71.1.800-806.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the ability of human coronaviruses to infect primary cultures of human neural cells. Double immunofluorescence with antibodies to virus and cell markers showed infection of fetal astrocytes and of adult microglia and astrocytes by strain OC43. RNA amplification revealed infection of fetal astrocytes, adult microglia, and a mixed culture of adult oligodendrocytes and astrocytes by strain 229E. Infectious virus was released only from fetal astrocytes, with higher titers for OC43. Human coronaviruses have the capacity to infect some cells of the central nervous system, although infection of adult cells appears abortive.

引用

收藏

页码：800 / 806

页数：7

相关论文

共 28 条

[1] 2 CORONAVIRUSES ISOLATED FROM CENTRAL NERVOUS-SYSTEM TISSUE OF 2 MULTIPLE-SCLEROSIS PATIENTS [J].

BURKS, JS ;

DEVALD, BL ;

JANKOVSKY, LD ;

GERDES, JC .

SCIENCE, 1980, 209 (4459) :933-934

[2]

Cabirac G F, 1995, Adv Exp Med Biol, V380, P79

[3] ENTRY OF CORONAVIRUS INTO PRIMATE CNS FOLLOWING PERIPHERAL INFECTION [J].

CABIRAC, GF ;

SOIKE, KF ;

ZHANG, JY ;

HOEL, K ;

BUTUNOI, C ;

CAI, GY ;

JOHNSON, S ;

MURRAY, RS .

MICROBIAL PATHOGENESIS, 1994, 16 (05) :349-357

[4] REGULATION OF VIRAL PERSISTENCE IN HUMAN GLIOBLASTOMA AND RHABDOMYOSARCOMA CELLS INFECTED WITH CORONAVIRUS OC43 [J].

COLLINS, AR ;

SORENSEN, O .

MICROBIAL PATHOGENESIS, 1986, 1 (06) :573-582

[5] ISOLATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR HUMAN C-MYC PROTO-ONCOGENE PRODUCT [J].

EVAN, GI ;

LEWIS, GK ;

RAMSAY, G ;

BISHOP, JM .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3610-3616

[6] INTERACTION OF MOUSE HEPATITIS-VIRUS (MHV) SPIKE GLYCOPROTEIN WITH RECEPTOR GLYCOPROTEIN MHVR IS REQUIRED FOR INFECTION WITH AN MHV STRAIN THAT EXPRESSES THE HEMAGGLUTININ-ESTERASE GLYCOPROTEIN [J].

GAGNETEN, S ;

GOUT, O ;

DUBOISDALCQ, M ;

ROTTIER, P ;

ROSSEN, J ;

HOLMES, KV .

JOURNAL OF VIROLOGY, 1995, 69 (02) :889-895

[7] SEQUENCE-ANALYSIS OF HUMAN CORONAVIRUS-229E MESSENGER RNA-4 AND MESSENGER RNA-5 - EVIDENCE FOR POLYMORPHISM AND HOMOLOGY WITH MYELIN BASIC-PROTEIN [J].

JOUVENNE, P ;

MOUNIR, S ;

STEWART, JN ;

RICHARDSON, CD ;

TALBOT, PJ .

VIRUS RESEARCH, 1992, 22 (02) :125-141

[8]

LAMARRE A, 1995, J IMMUNOL, V154, P3975

[9] DETECTION OF HUMAN T-LYMPHOTROPIC VIRUS TYPE-I (HTLV-I) TAX RNA IN THE CENTRAL-NERVOUS-SYSTEM OF HTLV-I-ASSOCIATED MYELOPATHY TROPICAL SPASTIC PARAPARESIS PATIENTS BY IN-SITU HYBRIDIZATION [J].

LEHKY, TJ ;

FOX, CH ;

KOENIG, S ;

LEVIN, MC ;

FLERLAGE, N ;

IZUMO, S ;

SATO, E ;

RAINE, CS ;

OSAME, M ;

JACOBSON, S .

ANNALS OF NEUROLOGY, 1995, 37 (02) :167-175

[10] SEQUENCE-ANALYSIS OF THE MEMBRANE-PROTEIN GENE OF HUMAN CORONAVIRUS OC43 AND EVIDENCE FOR O-GLYCOSYLATION [J].

MOUNIR, S ;

TALBOT, PJ .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2731-2736