Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

被引:32
作者
Romero Victorica, Matias [1 ]
Soria, Marcelo A. [2 ]
Alberto Batista-Garcia, Ramon [3 ]
Ceja-Navarro, Javier A. [4 ]
Vikram, Surendra [5 ]
Ortiz, Maximiliano [5 ]
Ontanon, Ornella [1 ]
Ghio, Silvina [1 ]
Martinez-Avila, Liliana [3 ]
Quintero Garcia, Omar Jasiel [3 ]
Etcheverry, Clara [6 ]
Campos, Eleonora [1 ]
Cowan, Donald [5 ]
Arneodo, Joel [1 ]
Talia, Paola M. [1 ]
机构
[1] Consejo Nacl Invest Cient & Tecnol CONICET, Inst Agrobiotecnol & Biol Mol IABIMO, Inst Nacl Tecnol Agr INTA, Buenos Aires, DF, Argentina
[2] Univ Buenos Aires, Fac Agron, Catedra Microbiol Agricola, INBA CONICET, Buenos Aires, DF, Argentina
[3] Univ Autonoma Estado Morelos, Inst Invest Ciencias Basicas & Aplicadas, Ctr Invest Dinam Celular, Cuernavaca, Morelos, Mexico
[4] Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA USA
[5] Univ Pretoria, Dept Biochem Genet & Microbiol, Ctr Microbial Ecol & Genom, Pretoria, South Africa
[6] Univ Nacl Nordeste, Biol Los Invertebrados, Fac Ciencias Exactas & Nat & Agrimensura, Corrientes, Argentina
关键词
GUT MICROBIOTA; GH10; XYLANASE; PHYLOGENETIC DIVERSITY; BIOCHEMICAL-CHARACTERIZATION; BIOCATALYTIC PROPERTIES; FUNCTIONAL-ANALYSIS; TREPONEMA-PRIMITIA; NITROGEN-FIXATION; LIGNOCELLULOSE; ENDO-BETA-1,4-XYLANASE;
D O I
10.1038/s41598-020-60850-5
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes. These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-beta-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 degrees C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.
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页数:14
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