High levels of expression of multiple enzymes in the Smirnoff-Wheeler pathway are important for high accumulation of ascorbic acid in acerola fruits

被引:14
作者
Suekawa, Marina [1 ]
Fujikawa, Yukichi [1 ]
Inoue, Akari [1 ]
Kondo, Takayuki [1 ,2 ]
Uchida, Eriko [3 ]
Koizumi, Takeshi [3 ]
Esaka, Muneharu [1 ]
机构
[1] Hiroshima Univ, Grad Sch Biosphere Sci, Higashihiroshima, Japan
[2] Kyushu Inst Technol, Dept Biosci & Bioinformat, Iizuka, Fukuoka, Japan
[3] Nichirei Corp, Corp Sci Res, Chiba, Japan
关键词
acerola; ascorbic acid; multiple expressions; the Smirnoff-Wheeler pathway; GENE-EXPRESSION; VITAMIN-C; DEHYDROASCORBATE; BIOSYNTHESIS; REDUCTASE;
D O I
10.1080/09168451.2019.1608808
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Acerola fruits contain abundant ascorbic acid (AsA). The gene expression levels of three upstream enzymes in the primary AsA biosynthesis pathway were correlated with AsA contents in the fruits of two acerola cultivars. Multiple overexpression of the enzymes increased AsA contents, suggesting their high expression is important for high AsA accumulation in acerola fruits and the breeding of AsA-rich plants.
引用
收藏
页码:1713 / 1716
页数:4
相关论文
共 10 条
  • [1] Gene expression of ascorbic acid biosynthesis related enzymes of the Smirnoff-Wheeler pathway in acerola (Malpighia glabra)
    Badejo, Adebanjo A.
    Fujikawa, Yukichi
    Esaka, Muneharu
    [J]. JOURNAL OF PLANT PHYSIOLOGY, 2009, 166 (06) : 652 - 660
  • [2] Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis
    Bulley, Sean M.
    Rassam, Maysoon
    Hoser, Dana
    Otto, Wolfgang
    Schuenemann, Nicole
    Wright, Michele
    MacRae, Elspeth
    Gleave, Andrew
    Laing, William
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 2009, 60 (03) : 765 - 778
  • [3] Gene expression of monodehydroascorbate reductase and dehydroascorbate reductase during fruit ripening and in response to environmental stresses in acerola (Malpighia glabra)
    Eltelib, Hani A.
    Badejo, Adebanjo A.
    Fujikawa, Yukichi
    Esaka, Muneharu
    [J]. JOURNAL OF PLANT PHYSIOLOGY, 2011, 168 (06) : 619 - 627
  • [4] L-Ascorbic acid metabolism during fruit development in an ascorbate-rich fruit crop chestnut rose (Rosa roxburghii Tratt)
    Huang, Ming
    Xu, Qiang
    Deng, Xiu-Xin
    [J]. JOURNAL OF PLANT PHYSIOLOGY, 2014, 171 (14) : 1205 - 1216
  • [5] An Upstream Open Reading Frame Is Essential for Feedback Regulation of Ascorbate Biosynthesis in Arabidopsis
    Laing, William A.
    Martinez-Sanchez, Marcela
    Wright, Michele A.
    Bulley, Sean M.
    Brewster, Di
    Dare, Andrew P.
    Rassam, Maysoon
    Wang, Daisy
    Storey, Roy
    Macknight, Richard C.
    Hellens, Roger P.
    [J]. PLANT CELL, 2015, 27 (03) : 772 - 786
  • [6] Strategies to increase vitamin C in plants: from plant defense perspective to food biofortification
    Locato, Vittoria
    Cimini, Sara
    De Gara, Laura
    [J]. FRONTIERS IN PLANT SCIENCE, 2013, 4
  • [7] Nagata M, 2013, J JPN SOC FOOD SCI, V60, P96, DOI 10.3136/nskkk.60.96
  • [8] Suekawa M., 2017, ASCORBIC ACID PLANT, P157
  • [9] Gene expression and promoter analysis of a novel tomato aldo-keto reductase in response to environmental stresses
    Suekawa, Marina
    Fujikawa, Yukichi
    Inada, Shuhei
    Murano, Asako
    Esaka, Muneharu
    [J]. JOURNAL OF PLANT PHYSIOLOGY, 2016, 200 : 35 - 44
  • [10] GDP-mannose 3′,5′-epimerase forms GDP-L-gulose, a putative intermediate for the de novo biosynthesis of vitamin C in plants
    Wolucka, BA
    Van Montagu, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (48) : 47483 - 47490