Lipopolysaccharide blocks induction of unfolded protein response in human hepatoma cell lines

被引：12

作者：

Jiang, Xia ^{[1
]}

Kanda, Tatsuo ^{[1
]}

Tanaka, Takeshi ^{[2
]}

Wu, Shuang ^{[1
]}

Nakamoto, Shingo ^{[3
]}

Imazeki, Fumio ^{[4
]}

Yokosuka, Osamu ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Gastroenterol & Nephrol, Chuo Ku, Chiba 2608670, Japan

[2] Chiba Univ, Grad Sch Med, Dept Environm Biochem, Chuo Ku, Chiba 2608670, Japan

[3] Chiba Univ, Grad Sch Med, Dept Mol Virol, Chuo Ku, Chiba 2608670, Japan

[4] Chiba Univ, Safety & Hlth Org, Inage Ku, Chiba 2638522, Japan

来源：

IMMUNOLOGY LETTERS | 2013年 / 152卷 / 01期

关键词：

PARP; GRP78; ER stress; Lipopolysaccharide; Unfolded protein response; HUMAN HEPATOCELLULAR-CARCINOMA; ENDOPLASMIC-RETICULUM STRESS; ER STRESS; INDUCED APOPTOSIS; ACTIVATION; GRP78; LPS; SORAFENIB; PATHWAY; CANCER;

D O I：

10.1016/j.imlet.2013.03.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In the present study, we examined whether unfolded protein response (UPR) determined the hepatic cell damage induced by an innate immune response including TLR signaling pathways. We observed that lipopolysaccharide (LPS) transcriptionally downregulates 78-kDa glucose-regulated protein/immunoglobulin heavy-chain binding protein (GRP78/Bip), known to confer resistance to apoptosis. We also observed that LPS blocked the induction of UPR and led to poly(ADP-ribose) polymerase (PARP) cleavage in hepatocytes. We also demonstrated that overexpression of GRP78 rescued HepG2 cells treated with LPS from PARP cleavage. These data suggest that UPR downregulation could be a collateral effect of the LPS treatment. We speculate that UPR is an important factor of hepatic cell damage induced by an innate immune response. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：8 / 15

页数：8

共 40 条

[1] Palmitoleate attenuates palmitate-induced Bim and PUMA up-regulation and hepatocyte lipoapoptosis [J].

Akazawa, Yuko ;

Cazanave, Sophie ;

Mott, Justin L. ;

Elmi, Nafisa ;

Bronk, Steven F. ;

Kohno, Shigeru ;

Charlton, Michael R. ;

Gores, Gregory J. .

JOURNAL OF HEPATOLOGY, 2010, 52 (04) :586-593

[2] Oncogenic Potential of Hepatitis C Virus Proteins [J].

Banerjee, Arup ;

Ray, Ratna B. ;

Ray, Ranjit .

VIRUSES-BASEL, 2010, 2 (09) :2108-2133

[3]

Chen R, 2011, FOLIA BIOL-PRAGUE, V57, P87

[4] Efficacy and safety of sorafenib in patients in the Asia-Pacific region with advanced hepatocellular carcinoma: a phase III randomised, double-blind, placebo-controlled trial [J].

Cheng, Ann-Lii ;

Kang, Yoon-Koo ;

Chen, Zhendong ;

Tsao, Chao-Jung ;

Qin, Shukui ;

Kim, Jun Suk ;

Luo, Rongcheng ;

Feng, Jifeng ;

Ye, Shenglong ;

Yang, Tsai-Sheng ;

Xu, Jianming ;

Sun, Yan ;

Liang, Houjie ;

Liu, Jiwei ;

Wang, Jiejun ;

Tak, Won Young ;

Pan, Hongming ;

Burock, Karin ;

Zou, Jessie ;

Voliotis, Dimitris ;

Guan, Zhongzhen .

LANCET ONCOLOGY, 2009, 10 (01) :25-34

[5] Glucose-Regulated Protein 78 Is a Novel Contributor to Acquisition of Resistance to Sorafenib in Hepatocellular Carcinoma [J].

Chiou, Jeng-Fong ;

Tai, Cheng-Jeng ;

Huang, Ming-Te ;

Wei, Po-Li ;

Wang, Yu-Huei ;

An, Jane ;

Wu, Chih-Hsiung ;

Liu, Tsan-Zon ;

Chang, Yu-Jia .

ANNALS OF SURGICAL ONCOLOGY, 2010, 17 (02) :603-612

[6] Lipopolysaccharide mediates endothelial apoptosis by a FADD-dependent pathway [J].

Choi, KB ;

Wong, F ;

Harlan, JM ;

Chaudhary, PM ;

Hood, L ;

Karsan, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (32) :20185-20188

[7] The ER stress pathway involving CHOP is activated in the lungs of LPS-treated mice [J].

Endo, M ;

Oyadomari, S ;

Suga, M ;

Mori, M ;

Gotoh, T .

JOURNAL OF BIOCHEMISTRY, 2005, 138 (04) :501-507

[8] Effects of lipopolysaccharide-stimulated inflammation and pyrazole-mediated hepatocellular injury on mouse hepatic Cyp2a5 expression [J].

Gilmore, WJ ;

Hartmann, G ;

Piquette-Miller, M ;

Marriott, J ;

Kirby, GM .

TOXICOLOGY, 2003, 184 (2-3) :211-226

[9]

Glimcher LH, 2011, CURR OPIN IMMUNOL, V23, P1, DOI 10.1016/j.coi.2010.12.001

[10] Stress management at the ER: Regulators of ER stress-induced apoptosis [J].

Gorman, Adrienne M. ;

Healy, Sandra J. M. ;

Jaeger, Richard ;

Samali, Afshin .

PHARMACOLOGY & THERAPEUTICS, 2012, 134 (03) :306-316

← 1 2 3 4 →