Stimulative role of ST6GALNAC1 in proliferation, migration and invasion of ovarian cancer stem cells via the Akt signaling pathway

被引:26
作者
Wang, Wen-Yan [1 ,2 ]
Cao, Yun-Xia [2 ]
Zhou, Xiao [3 ]
Wei, Bing [1 ]
Zhan, Lei [1 ]
Sun, Shi-Ying [1 ]
机构
[1] Anhui Med Univ, Hosp 2, Dept Obstet & Gynecol, Hefei 230601, Anhui, Peoples R China
[2] Anhui Med Univ, Teaching & Res Grp Obstet & Gynecol, 81 Meishan Rd, Hefei 230032, Anhui, Peoples R China
[3] Anhui Med Univ, Hosp 2, Dept Cardiothorac Surg, Hefei 230601, Anhui, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
Ovarian cancer; ST6GALNAC1; Akt signaling pathway; Ovarian cancer stem cells; Proliferation; Migration; Invasion; Tumorigenicity; Tumorsphere; THERAPEUTIC TARGET; DOWN-REGULATION; EXPRESSION; RECEPTOR; MARKERS; NANOG; ADHESION; CD44;
D O I
10.1186/s12935-019-0780-7
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
BackgroundOvarian cancer is known as one of the most common cancers in the world among women. ST6GALNAC1 is highly expressed in cancer stem cells (CSCs), which correlates to high tumor-initiating, self-renewal and differentiation abilities. This present study aims to investigate how ST6GALNAC1 affects ovarian cancer stem cells (OCSCs).MethodsIn order to identify the differentially expressed genes related to ovarian cancer, microarray-based gene expression profiling of ovarian cancer was used, and ST6GALANC1 was one of the identified targets. After that, levels of ST6GALNAC1 in OCSCs and ovarian cancer cells were examined. Subsequently, an Akt signaling pathway inhibitor LY294002 was introduced into the cluster of differentiation 90(+) (CD90(+)) stem cells, and cell proliferation, migration and invasion, levels of CXCL16, EGFR, CD44, Nanog and Oct4, as well as tumorigenicity of OCSCs were examined.ResultsBy using a comprehensive microarray analysis, it was determined that ST6GALNAC1 was highly expressed in ovarian cancer and it regulated the Akt signaling pathway. High levels of ST6GALNAC1 were observed in OCSCs and ovarian cancer cells. Silencing ST6GALNAC1 was shown to be able to reduce cell proliferation, migration, invasion, self-renewal ability, tumorigenicity of OCSCs. In accordance with these results, the effects of ST6GALNAC1 in OCSCs were dependent on the Akt signaling pathway.ConclusionsWhen taken together, our findings defined the potential stimulative roles of ST6GALNAC1 in ovarian cancer and OCSCs, which relied on the Akt signaling pathway.
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页数:17
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