Surface Functionality of Nanoparticles Determines Cellular Uptake Mechanisms in Mammalian Cells

被引：174

作者：

Saha, Krishnendu ^{[1
]}

Kim, Sung Tae ^{[1
]}

Yan, Bo ^{[1
]}

Miranda, Oscar R. ^{[1
]}

Alfonso, Felix S. ^{[1
]}

Shlosman, Denis ^{[1
]}

Rotello, Vincent M. ^{[1
]}

机构：

[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA

来源：

SMALL | 2013年 / 9卷 / 02期

基金：

美国国家卫生研究院;

关键词：

gold nanoparticles; surface monolayers; uptake mechanisms; caveolae; dynamin-dependent endocytosis; RECEPTOR-MEDIATED ENDOCYTOSIS; CLATHRIN-INDEPENDENT ENDOCYTOSIS; COATED GOLD NANOPARTICLES; TARGETED DRUG-DELIVERY; SCAVENGER RECEPTORS; LIVING CELLS; SIZE; INTERNALIZATION; PATHWAYS; MACROPHAGES;

D O I：

10.1002/smll.201201129

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nanoparticles (NPs) are versatile scaffolds for numerous biomedical applications including drug delivery and bioimaging. The surface functionality of NPs essentially dictates intracellular NP uptake and controls their therapeutic action. Using several pharmacological inhibitors, it is demonstrated that the cellular uptake mechanisms of cationic gold NPs in both cancer (HeLa) and normal cells (MCF10A) strongly depend on the NP surface monolayer, and mostly involve caveolae and dynamin-dependent pathways as well as specific cell surface receptors (scavenger receptors). Moreover, these NPs show different uptake mechanisms in cancer and normal cells, providing an opportunity to develop NPs with improved selectivity for delivery applications.

引用

页码：300 / 305

页数：6

共 60 条

[1] Short Ligands Affect Modes of QD Uptake and Elimination in Human Cells
Al-Hajaj, Noura A.
Moquin, Alexandre
Neibert, Kevin D.
Soliman, Ghareb M.
Winnik, Francoise M.
Maysinger, Dusica
[J]. ACS NANO, 2011, 5 (06) : 4909 - 4918
[2] Cellular Uptake and Cytotoxicity of Gold Nanorods: Molecular Origin of Cytotoxicity and Surface Effects
Alkilany, Alaaldin M.
Nagaria, Pratik K.
Hexel, Cole R.
Shaw, Timothy J.
Murphy, Catherine J.
Wyatt, Michael D.
[J]. SMALL, 2009, 5 (06) : 701 - 708
[3] A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages
Araki, N
Johnson, MT
Swanson, JA
[J]. JOURNAL OF CELL BIOLOGY, 1996, 135 (05) : 1249 - 1260
[4] Effect of mechanical properties of hydrogel nanoparticles on macrophage cell uptake
Banquy, Xavier
Suarez, Fernando
Argaw, Anteneh
Rabanel, Jean-Michel
Grutter, Peter
Bouchard, Jean-Francois
Hildgen, Patrice
Giasson, Suzanne
[J]. SOFT MATTER, 2009, 5 (20) : 3984 - 3991
[5] Endocytic mechanisms for targeted drug delivery
Bareford, Lisa A.
Swaan, Peter W.
[J]. ADVANCED DRUG DELIVERY REVIEWS, 2007, 59 (08) : 748 - 758
[6] Switching the Targeting Pathways of a Therapeutic Antibody by Nanodesign
Bhattacharyya, Sanjib
Singh, Raman Deep
Pagano, Richard
Robertson, J. David
Bhattacharya, Resham
Mukherjee, Priyabrata
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (07) : 1563 - 1567
[7] Quantitative Evaluation of Cellular Uptake and Trafficking of Plain and Polyethylene Glycol-Coated Gold Nanoparticles
Brandenberger, Christina
Muehlfeld, Christian
Ali, Zulqurnain
Lenz, Anke-Gabriele
Schmid, Otmar
Parak, Wolfgang J.
Gehr, Peter
Rothen-Rutishauser, Barbara
[J]. SMALL, 2010, 6 (15) : 1669 - 1678
[8] A novel type of PTD, common helix-loop-helix motif, could efficiently mediate protein transduction into mammalian cells
Chen, Jing
Li, Ge
Lu, Jun
Chen, Lei
Huang, Yin
Wu, Huiling
Zhang, Jiaxin
Lu, Daru
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 347 (04) : 931 - 940
[9] Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes
Chithrani, B. Devika
Chan, Warren C. W.
[J]. NANO LETTERS, 2007, 7 (06) : 1542 - 1550
[10] Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells
Chithrani, BD
Ghazani, AA
Chan, WCW
[J]. NANO LETTERS, 2006, 6 (04) : 662 - 668

← 1 2 3 4 5 6 →