Differential expression of semaphorin 3A and its receptors during mouse retinal development

被引：3

作者：

Ko, Ji-Ae ^{[1
]}

Mizuno, Yukari ^{[2
]}

Shibasaki, Momoko ^{[1
]}

Yamane, Ken ^{[1
]}

Chikama, Tai-ichiro ^{[1
]}

Sonoda, Koh-Hei ^{[2
]}

Kiuchi, Yoshiaki ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Dept Ophthalmol, Hiroshima 7348551, Japan

[2] Yamaguchi Univ, Grad Sch Med, Dept Ophthalmol, Ube, Yamaguchi 755, Japan

来源：

CELL BIOCHEMISTRY AND FUNCTION | 2012年 / 30卷 / 07期

基金：

日本学术振兴会;

关键词：

retina; development; semaphorin; 3A; neuropilin; 1; plexin A; CHEMOTROPIC RESPONSES; CORNEAL FIBROBLASTS; GROWTH; TRANSMEMBRANE; NEUROPILIN-1; PROTEIN; DISRUPTION; COLLAPSE; FAMILY; CELLS;

D O I：

10.1002/cbf.2833

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Semaphorins not only function in axon guidance during development but also contribute to various other biological processes. We have now examined the expression of semaphorin 3A (Sema3A) and its receptor components neuropilin 1 (Npn1) and plexin A (PlxA) during development of the mouse retina. Immunohistofluorescence analysis revealed that the expression patterns of Sema3A and Npn1 were similar during embryonic and postnatal development. The expression pattern of PlxA was also similar to those of Sema3A and Npn1 during embryonic and early postnatal (before eye opening) developments. However, the pattern of PlxA expression changed markedly after eye opening, with the expression disappearing from the optic nerve and increasing in intensity in the retinal pigment epithelium. Immunoprecipitation analysis showed that Sema3A interacted with PlxA in the retinal pigment epithelial cell line ARPE19 but not in the retinal ganglion cell line RGC5, whereas the opposite pattern of association was apparent for Sema3A and Npn1. Given that atmospheric oxygen is thought to play a role in the differentiation and maintenance of various ocular cell types, our results suggest that Sema3APlxA signalling activated by an effect of ambient oxygen on PlxA expression may contribute to differentiation of the retinal pigment epithelium. Copyright (c) 2012 John Wiley & Sons, Ltd.

引用

页码：563 / 568

页数：6

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