Vessel-Specific Reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in Dwarfed ccr1 Mutants Restores Vessel and Xylary Fiber Integrity and Increases Biomass

被引:75
作者
De Meester, Barbara [1 ,2 ]
de Vries, Lisanne [1 ,2 ]
Ozparpucu, Merve [3 ,4 ]
Gierlinger, Notburga [5 ]
Corneillie, Sander [1 ,2 ]
Pallidis, Andreas [1 ,2 ]
Goeminne, Geert [1 ,2 ,6 ]
Morreel, Kris [1 ,2 ,6 ]
De Bruyne, Michiel [1 ,2 ]
De Rycke, Riet [1 ,2 ]
Vanholme, Ruben [1 ,6 ]
Boerjan, Wout [1 ,2 ,6 ]
机构
[1] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium
[2] VIB Ctr Plant Syst Biol, B-9052 Ghent, Belgium
[3] Swiss Fed Inst Technol Zurich, Inst Bldg Mat, CH-8093 Zurich, Switzerland
[4] Swiss Fed Labs Mat Sci & Technol, Appl Wood Mat, CH-8600 Dubendorf, Switzerland
[5] Univ Nat Resources & Life Sci Vienna, Inst Biophys, A-1190 Vienna, Austria
[6] VIB Metabol Core, B-9052 Ghent, Belgium
关键词
LIGNIN BIOSYNTHESIS PERTURBATIONS; SECONDARY WALL FORMATION; SHIKIMATE-ESTERASE CSE; DOWN-REGULATION; SACCHARIFICATION YIELD; FERULIC ACID; CELL-WALLS; ARABIDOPSIS; LIGNIFICATION; DEFICIENT;
D O I
10.1104/pp.17.01462
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Lignocellulosic biomass is recalcitrant toward deconstruction into simple sugars due to the presence of lignin. To render lignocellulosic biomass a suitable feedstock for the bio-based economy, plants can be engineered to have decreased amounts of lignin. However, engineered plants with the lowest amounts of lignin exhibit collapsed vessels and yield penalties. Previous efforts were not able to fully overcome this phenotype without settling in sugar yield upon saccharification. Here, we reintroduced CINNAMOYL-COENZYME A REDUCTASE1 (CCR1) expression specifically in the protoxylem and metaxylem vessel cells of Arabidopsis (Arabidopsis thaliana) ccr1 mutants. The resulting ccr1 ProSNBE: CCR1 lines had overcome the vascular collapse and had a total stem biomass yield that was increased up to 59% as compared with the wild type. Raman analysis showed that monolignols synthesized in the vessels also contribute to the lignification of neighboring xylary fibers. The cell wall composition and metabolome of ccr1 ProSNBE: CCR1 still exhibited many similarities to those of ccr1 mutants, regardless of their yield increase. In contrast to a recent report, the yield penalty of ccr1 mutants was not caused by ferulic acid accumulation but was (largely) the consequence of collapsed vessels. Finally, ccr1 ProSNBE: CCR1 plants had a 4-fold increase in total sugar yield when compared with wild-type plants.
引用
收藏
页码:611 / 633
页数:23
相关论文
共 87 条
  • [31] Lignocellulosic biomass: a sustainable platform for the production of bio-based chemicals and polymers
    Isikgor, Furkan H.
    Becer, C. Remzi
    [J]. POLYMER CHEMISTRY, 2015, 6 (25) : 4497 - 4559
  • [32] Improving Saccharification Efficiency of Alfalfa Stems Through Modification of the Terminal Stages of Monolignol Biosynthesis
    Jackson, Lisa A.
    Shadle, Gail L.
    Zhou, Rui
    Nakashima, Jin
    Chen, Fang
    Dixon, Richard A.
    [J]. BIOENERGY RESEARCH, 2008, 1 (3-4) : 180 - 192
  • [33] Cloning and characterization of irregular xylem4 (irx4):: a severely lignin-deficient mutant of Arabidopsis
    Jones, L
    Ennos, AR
    Turner, SR
    [J]. PLANT JOURNAL, 2001, 26 (02) : 205 - 216
  • [34] Transcription switches for protoxylem and metaxylem vessel formation
    Kubo, M
    Udagawa, M
    Nishikubo, N
    Horiguchi, G
    Yamaguchi, M
    Ito, J
    Mimura, T
    Fukuda, H
    Demura, T
    [J]. GENES & DEVELOPMENT, 2005, 19 (16) : 1855 - 1860
  • [35] The Arabidopsis cinnamoyl CoA reductase irx4 mutant has a delayed but coherent (normal) program of lignification
    Laskar, Dhrubojyoti D.
    Jourdes, Michael
    Patten, Ann M.
    Helms, Gregory L.
    Davin, Laurence B.
    Lewis, Norman G.
    [J]. PLANT JOURNAL, 2006, 48 (05) : 674 - 686
  • [36] Downregulation of cinnamoyl-coenzyme a reductase in poplar:: Multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure
    Leple, Jean-Charles
    Dauwe, Rebecca
    Morreel, Kris
    Storme, Veronique
    Lapierre, Catherine
    Pollet, Brigitte
    Naumann, Annette
    Kang, Kyu-Young
    Kim, Hoon
    Ruel, Katia
    Lefebvre, Andree
    Joseleau, Jean-Paul
    Grima-Pettenati, Jacqueline
    De Rycke, Riet
    Andersson-Gunneras, Sara
    Erban, Alexander
    Fehrle, Ines
    Petit-Conil, Michel
    Kopka, Joachim
    Polle, Andrea
    Messens, Eric
    Sundberg, Bjorn
    Mansfield, Shawn D.
    Ralph, John
    Pilate, Gilles
    Boerjan, Wout
    [J]. PLANT CELL, 2007, 19 (11) : 3669 - 3691
  • [37] The Class II KNOX gene KNAT7 negatively regulates secondary wall formation in Arabidopsis and is functionally conserved in Populus
    Li, Eryang
    Bhargava, Apurva
    Qiang, Weiya
    Friedmann, Michael C.
    Forneris, Natascha
    Savidge, Rodney A.
    Johnson, Lee A.
    Mansfield, Shawn D.
    Ellis, Brian E.
    Douglas, Carl J.
    [J]. NEW PHYTOLOGIST, 2012, 194 (01) : 102 - 115
  • [38] Designed for deconstruction - poplar trees altered in cell wall lignification improve the efficacy of bioethanol production
    Mansfield, Shawn D.
    Kang, Kyu-Young
    Chapple, Clint
    [J]. NEW PHYTOLOGIST, 2012, 194 (01) : 91 - 101
  • [39] Waterproofing in Arabidopsis: Following Phenolics and Lipids In situ by Confocal Raman Microscopy
    Mateu, Batirtze Prats
    Hauser, Marie Theres
    Heredia, Antonio
    Gierlinger, Notburga
    [J]. FRONTIERS IN CHEMISTRY, 2016, 4
  • [40] Secondary wall NAC binding element (SNBE), a key cis-acting element required for target gene activation by secondary wall NAC master switches
    McCarthy, Ryan L.
    Zhong, Ruiqin
    Ye, Zheng-Hua
    [J]. PLANT SIGNALING & BEHAVIOR, 2011, 6 (09) : 1282 - 1285