Nitric oxide inhibitors with a spiro diterpenoid skeleton from Scutellaria formosana: Structures, NO inhibitory effects, and interactions with iNOS

被引：19

作者：

Wang, Peixia ^{[1
,2
]}

Yang, Xueyuan ^{[1
,2
]}

Liu, Feng ^{[1
,2
]}

Liang, Yue ^{[1
,2
]}

Su, Guochen ^{[1
,2
]}

Tuerhong, Muhetaer ^{[3
]}

Jin, Da-Qing ^{[4
]}

Xu, Jing ^{[1
,2
]}

Lee, Dongho ^{[5
]}

Ohizumi, Yasushi ^{[6
]}

Guo, Yuanqiang ^{[1
,2
]}

机构：

[1] Nankai Univ, State Key Lab Med Chem Biol, Coll Pharm, Tianjin 300350, Peoples R China

[2] Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300350, Peoples R China

[3] Kashgar Univ, Coll Chem & Environm Sci, Lab Xinjiang Native Med & Edible Plant Resources, Kashgar 844000, Peoples R China

[4] Nankai Univ, Sch Med, Tianjin 300071, Peoples R China

[5] Korea Univ, Coll Life Sci & Biotechnol, Dept Biosyst & Biotechnol, Seoul 02841, South Korea

[6] Univ Shizuoka, Sch Pharmaceut Sci, Dept Med Biochem, Shizuoka, Japan

来源：

BIOORGANIC CHEMISTRY | 2018年 / 76卷

基金：

中国国家自然科学基金;

关键词：

NO inhibitors; Scutellaria formosana; Spiro diterpenoid; Molecular docking; iNOS; CYTOTOXIC NEOCLERODANE DITERPENOIDS; AERIAL PARTS; BARBATA; PHYTOCHEMICALS; TRITERPENOIDS; TWIGS;

D O I：

10.1016/j.bioorg.2017.10.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phytochemical investigation to obtain new NO inhibitors resulted in the isolation of five new spiro diterpenoids (1 - 5) from the aerial parts of Scutellaria formosana. The structures were elucidated on the basis of extensive 1D and 2D NMR spectroscopic data analysis, and the absolute configurations of these compounds were established via comparison of experimental and calculated electronic circular dichroism (ECD) spectra. The nitric oxide (NO) inhibitory effects were evaluated and all of the compounds showed inhibitory effects on lipopolysaccharide-induced NO production in murine microglial BV-2 cells. The possible mechanism of NO inhibition of bioactive compounds was also investigated using molecular docking, which revealed the interactions of bioactive compounds with the iNOS protein. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：53 / 60

页数：8

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