Spontaneous rearrangements in RNA sequences

被引：62

作者：

Chetverina, HV ^{[1
]}

Demidenko, AA ^{[1
]}

Ugarov, VI ^{[1
]}

Chetverin, AB ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Prot Res, Pushchino 142292, Moscow Region, Russia

来源：

FEBS LETTERS | 1999年 / 450卷 / 1-2期

基金：

俄罗斯基础研究基金会;

关键词：

RNA recombination; transesterification reaction; Q beta phage satellite RNA; Q beta replicase; molecular colony technique;

D O I：

10.1016/S0014-5793(99)00469-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of RNAs to spontaneously rearrange their sequences under physiological conditions is demonstrated using the molecular colony technique, mhich allows single RNA molecules to be detected provided that they are amplifiable by the replicase of bacteriophage Q beta, The rearrangements are Mg2+-dependent, sequence-non-specific, and occur both in trans and in cis at a rate of 10(-9) h(-1) per site, The results suggest that the mechanism of spontaneous RNA rearrangements differs from the transesterification reactions earlier observed in the presence of Q beta replicase, and have a number of biologically important implications. (C) 1999 Federation of European Biochemical Societies.

引用

页码：89 / 94

页数：6

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