Upregulation of Long Noncoding RNA SPRY4-IT1 Modulates Proliferation, Migration, Apoptosis, and Network Formation in Trophoblast Cells HTR-8SV/neo

被引：84

作者：

Zou, Yanfen ^{[1
]}

Jiang, Ziyan ^{[1
]}

Yu, Xiang ^{[2
]}

Sun, Ming ^{[2
]}

Zhang, Yuanyuan ^{[1
]}

Zuo, Qing ^{[1
]}

Zhou, Jing ^{[2
]}

Yang, Nana ^{[1
]}

Han, Ping ^{[1
]}

Ge, Zhiping ^{[1
]}

De, Wei ^{[2
]}

Sun, Lizhou ^{[1
]}

机构：

[1] Nanjing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Nanjing, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Nanjing, Jiangsu, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 11期

基金：

中国国家自然科学基金;

关键词：

ANTIANGIOGENIC FACTORS; NORMAL-PREGNANCY; PREECLAMPSIA; GENE; EXPRESSION; PATHOGENESIS; LIFE;

D O I：

10.1371/journal.pone.0079598

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

SPRY4-IT1 has been reported to have extremely high expression in normal placenta tissues. It is a Long noncoding RNA (lncRNA), which is associated with cell growth, migration, invasion, and apoptosis in melanoma. A 2.8-fold increase of SPRY4-IT1 expression was validated by Real-time reverse transcription-polymerase chain reaction (qRT-PCR) in severe preeclamptic placenta as compared with that of the normal ones (n=25) in this study. Furthermore, the role of SPRY4-IT1 in proliferation, migration, apoptosis, and network formation ability of trophoblast cells HTR-8/SVneo was assessed. Suppression of SPRY4-IT1 using siRNA treatment and its overexpression using plasmid targeting SPRY4-IT1 were performed in order to explore the biological function of SPRY4-IT1 in the development and progression of trophoblast cells HTR-8/SVneo, in vitro. The results showed that SPRY4-IT1 knockdown enhanced the cell migration and proliferation, and reduced the response of cells to apoptosis. However, exogenous SPRY4-IT1 overexpression significantly decreased the cell migration and proliferation, while increased cell apoptosis. Our study showed for the first time that aberrant expression of lncRNA SPRY4-IT1 might contribute to the abnormal condition of trophoblast cells HTR-8/SVneo. Therefore, we proposed SPRY4-IT1 as a novel lncRNA molecule, which might be associated with the pathogenesis of preeclampsia and might provide a new target for its early diagnosis and treatment.

引用

页数：10

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