Overexpression of ZDHHC14 promotes migration and invasion of scirrhous type gastric cancer

被引：31

作者：

Oo, Htoo Zarni ^{[1
]}

Sentani, Kazuhiro ^{[1
]}

Sakamoto, Naoya ^{[1
]}

Anami, Katsuhiro ^{[1
]}

Naito, Yutaka ^{[1
]}

Uraoka, Naohiro ^{[1
]}

Oshima, Takashi ^{[2
]}

Yanagihara, Kazuyoshi ^{[3
]}

Oue, Naohide ^{[1
]}

Yasui, Wataru ^{[1
]}

机构：

[1] Hiroshima Univ, Inst Biomed & Hlth Sci, Dept Mol Pathol, Hiroshima 7348551, Japan

[2] Yokohama City Univ, Med Ctr, Gastroenterol Ctr, Yokohama, Kanagawa 2320024, Japan

[3] Natl Canc Ctr Hosp East, Exploratory Oncol & Clin Trial Ctr, Div Translat Res, Kashiwa, Chiba 2778577, Japan

来源：

ONCOLOGY REPORTS | 2014年 / 32卷 / 01期

关键词：

gastric cancer; CAST; ZDHHC14; DHHC domain-containing; protein; 14; invasion; GENE-EXPRESSION; SERIAL ANALYSIS; UP-REGULATION; CELL-LINES; REG-IV; CARCINOMA; PALMITOYLATION; LOCALIZATION; METASTASIS; REDUCTION;

D O I：

10.3892/or.2014.3166

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Scirrhous type gastric cancer is highly aggressive and has a poorer prognosis than many other types of gastric carcinoma, due to its characteristic rapid cancer cell infiltration and proliferation, extensive stromal fibrosis, and frequent peritoneal dissemination. The aim of the present study was to identify novel prognostic markers or therapeutic targets for scirrhous type gastric cancer. We reviewed a list of genes with upregulated expression in scirrhous type gastric cancer and compared their expression with that in normal stomach from our previous Escherichia coli (E. coli) ampicillin secretion-trap (CAST) analysis. We focused on the ZDHHC14 gene, which encodes zinc finger, DHHC-type containing 14 protein. qRT-PCR analysis of ZDHHC14 in 41 gastric cancer cases revealed that compared to mRNA levels in normal non-neoplastic gastric mucosa, ZDHHC14 mRNA was overexpressed in 27% of gastric cancer tissue samples. The overexpression of ZDHHC14 was significantly associated with depth of tumor invasion, undifferentiated histology and scirrhous pattern. The invasiveness of ZDHHC14-knockdown HSC-44PE and 44As3 gastric cancer cells was decreased in comparison with that of the negative control siRNA-transfected cells, together with downregulation of MMP-17 mRNA. Integrins alpha 5 and beta 31 were also downregulated in ZDHHC14-knockdown 44As3 cells. Forced expression of ZDHHC14 activated gastric cancer cell migration and invasion in vitro. These results indicate that ZDHHC14 is involved in tumor progression in patients with scirrhous type gastric cancer.

引用

页码：403 / 410

页数：8

共 32 条

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