Class 1 integron-borne cassettes harboring blaCARB-2 gene in multidrug-resistant and virulent Salmonella Typhimurium ST19 strains recovered from clinical human stool samples, United States

被引：16

作者：

Monte, Daniel F. M. ^{[1
,2
]}

Sellera, Fabio P. ^{[3
]}

Lopes, Ralf ^{[4
]}

Keelara, Shivaramu ^{[2
]}

Landgraf, Mariza ^{[1
]}

Greene, Shermalyn ^{[5
]}

Fedorka-Cray, Paula J. ^{[2
]}

Thakur, Siddhartha ^{[2
,6
]}

机构：

[1] Univ Sao Paulo, Fac Pharmaceut Sci, Food Res Ctr, Dept Food & Expt Nutr, Sao Paulo, Brazil

[2] North Carolina State Univ, Coll Vet Med, Dept Populat Hlth & Pathobiol, Raleigh, NC 27695 USA

[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo, Brazil

[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto, Brazil

[5] Dept Hlth & Human Serv, Mol Diagnost & Epidemiol Lab Unit, State Lab Publ Hlth, Div Publ Hlth, Raleigh, NC USA

[6] North Carolina State Univ, Comparat Med Inst, Raleigh, NC 27695 USA

来源：

PLOS ONE | 2020年 / 15卷 / 10期

基金：

美国国家卫生研究院;

关键词：

ENTERICA SEROVAR TYPHIMURIUM; ANTIMICROBIAL RESISTANCE; ANTIBIOTIC-RESISTANCE; PIGS; EMERGENCE; VARIANTS; SEQUENCE; PLASMID; SPREAD; MIG-14;

D O I：

10.1371/journal.pone.0240978

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

International lineages, such as Salmonella Typhimurium sequence type (ST) 19, are most often associated with foodborne diseases and deaths in humans. In this study, we compared the whole-genome sequences of five S. Typhimurium strains belonging to ST19 recovered from clinical human stool samples in North Carolina, United States. Overall, S. Typhimurium strains displayed multidrug-resistant profile, being resistance to critically and highly important antimicrobials including ampicillin, ticarcillin/clavulanic acid, streptomycin and sulfisoxazole, chloramphenicol, tetracycline, respectively. Interestingly, all S. Typhimurium strains carried class 1 integron (intl1) and we were able to describe two genomic regions surrounding bla(CARB-2) gene, size 4,062 bp and 4,422 bp for S. Typhimurium strains (HS5344, HS5437, and HS5478) and (HS5302 and HS5368), respectively. Genomic analysis for antimicrobial resistome confirmed the presence of clinically important genes, including bla(CARB-2), aac(6')-Iaa, aadA2b, sul1, tetG, floR, and biocide resistance genes (qacE Delta 1). S. Typhimurium strains harbored IncFIB plasmid containing spvRABCD operon, as well as rck and pef virulence genes, which constitute an important apparatus for spreading the virulence plasmid. In addition, we identified several virulence genes, chromosomally located, while the phylogenetic analysis revealed clonal relatedness among these strains with S. enterica isolated from human and non-human sources obtained in European and Asian countries. Our results provide new insights into this unusual class 1 integron in virulent S. Typhimurium strains that harbors a pool of genes acting as potential hotspots for horizontal gene transfer providing readily adaptation to new surrounds, as well as being crucially required for virulence in vivo. Therefore, continuous genomic surveillance is an important tool for safeguarding human health.

引用

页数：11