A Chinese White Pear (Pyrus bretschneideri) BZR Gene PbBZR1 Act as a Transcriptional Repressor of Lignin Biosynthetic Genes in Fruits

BZR transcription factors play essential roles in plant growth and environmental stimuli, and they are also the positive regulators of Brassinosteroid (BR) signal transduction in diverse plants. In addition, BZR TFs, as crucial regulators of BR synthesis, may have multiple stress-resistance functions and their related regulatory mechanisms have been well illustrated in model plants. Here, we carried out a genome-wide identification ofBZRmembers in Chinese pear (Pyrus bretschneideri) and identified 13 members. By comparative analysis in five Rosaceae genomes,BZRmembers in the pear genome may have undergone large-scale duplication events during evolution. Purifying selection played an important role in almost all of the orthologous and paralogous gene pairs. According to the expression analysis of thePbBZRsduring fruit development, threePbBZRswere selected for detailed analysis. Transcriptional activation assays presented thatPbBZR1repressed the promoters ofP. bretschneiderilignin biosynthetic genes, such asPbCES9,PbCOMT3, andPbHCT6. Our study traces the evolution ofBZRgene family members in Rosaceae genomes and illustrates that the rates of gene loss and gain are far from equilibrium in different species. At the same time, our results suggest thatPbBZR1may be involved in the negative regulation of lignin biosynthesis.