Iron and citrate export by a major facilitator superfamily pump regulates metabolism and stress resistance in Salmonella Typhimurium

被引：83

作者：

Frawley, Elaine R. ^{[1
]}

Crouch, Marie-Laure V. ^{[1
]}

Bingham-Ramos, Lacey K. ^{[1
]}

Robbins, Hannah F. ^{[1
]}

Wang, Wenliang ^{[3
]}

Wright, Gerard D. ^{[3
]}

Fang, Ferric C. ^{[1
,2
]}

机构：

[1] Univ Washington, Dept Lab Med, Seattle, WA 98195 USA

[2] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

[3] McMaster Univ, Michael G DeGroote Inst Infect Dis Res, Hamilton, ON L8S 4L8, Canada

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 29期

基金：

美国国家卫生研究院;

关键词：

antibiotic resistance; oxidative stress; ENTERICA SEROVAR TYPHIMURIUM; ESCHERICHIA-COLI; BACTERICIDAL ANTIBIOTICS; REACTIVE OXYGEN; CELL-DEATH; OXIDATIVE DAMAGE; SULFUR CLUSTERS; NITRIC-OXIDE; GENES; MULTIDRUG;

D O I：

10.1073/pnas.1218274110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The efficacy of antibiotics and host defenses has been linked to the metabolic and redox states of bacteria. In this study we report that a stress-induced export pump belonging to the major facilitator superfamily effluxes citrate and iron from the enteric pathogen Salmonella Typhimurium to arrest growth and ameliorate the effects of antibiotics, hydrogen peroxide, and nitric oxide. The transporter, formerly known as MdtD, is now designated IceT (iron citrate efflux transporter). Iron efflux via an iron-chelating tricarboxylic acid cycle intermediate provides a direct link between aerobic metabolism and bacterial stress responses, representing a unique mechanism of resistance to host defenses and antimicrobial agents of diverse classes.

引用

页码：12054 / 12059

页数：6

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