Protective effect of nordihydroguaiaretic acid (NDGA) against norgestrel induced genotoxic damage

被引：19

作者：

Siddique, YH ^{[1
]}

Beg, T ^{[1
]}

Afzal, M ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Fac Life Sci, Dept Zool, Genet Sect, Aligarh 202002, Uttar Pradesh, India

来源：

TOXICOLOGY IN VITRO | 2006年 / 20卷 / 02期

关键词：

nordihydroguaiaretic acid; norgestrel; human lymphocytes; antioxidants; genotoxicity;

D O I：

10.1016/j.tiv.2005.06.027

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Nordihydroguaiaretic acid (NDGA) is a phenolic lignan and possesses antioxidant and number of properties potentially useful to man. The effect of NDGA was studied against norgestrel induced genotoxic damage, using sister chromatid exchanges (SCEs), chromosomal aberrations (CAs), mitotic index (MI) and replication index (RI) as parameters. Amounts of 5, 10 and 20 mu M of norgestrel was tested for its genotoxic effect in the absence as well as presence of S9 mix, and was found to be genotoxic at 10 and 20 mu M in the presence of S9 mix. Again, 10 mu M of norgestrel was treated with 0.5 and 1 mu M of NDGA, separately, in the presence of S9 mix. Similar treatment was given with 20 mu M of norgestrel. Treatments given with NDGA result in the reduction of SCE, CA and increase of MI as well as RI, suggesting its protective action on human lymphocytes in vitro against the norgestrel induced genotoxic damage. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：227 / 233

页数：7

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