Isolation and characterization of 20 polymorphic microsatellites loci forXenocypris davidibased on high-throughput sequencing

被引：6

作者：

Guo, Aihuan ^{[1
]}

Yuan, Julin ^{[1
]}

Lian, Qingping ^{[1
]}

Li, Ming ^{[2
]}

Gu, Zhimin ^{[1
]}

机构：

[1] Minist Agr, Key Lab Hlth Freshwater Aquaculture,Zhejiang Inst, Key Lab Freshwater Aquaculture Genet & Breeding Z, Zhejiang Res Ctr,East China Sea Fishery Res Inst, 999th South Hangchangqiao Rd, Huzhou 313000, Peoples R China

[2] Jinhua Fisheries Technol Extens Stn, Jinhua 321000, Zhejiang, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2020年 / 47卷 / 10期

关键词：

Xenocypris davidi; Polymorphic; Genetic diversity; Simple sequence repeat (SSR) markers; Primer development; Cyprinidae; GENETIC DIVERSITY; NULL ALLELES; MARKERS; LINNAEUS;

D O I：

10.1007/s11033-020-05834-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Xenocypris davidiis one of the most economically important freshwater fish in China. However, few molecular markers have been reported for this species, impeding in-depth population genetic, dispersal, and gene flow studies. In the present study, a batch of novel polymorphic microsatellites from the genome ofX. davidiwere isolated and characterized using high-throughput sequencing. A total of 20 microsatellite markers were isolated. Analysis of 33 individuals revealed an average of 7.35 alleles per locus, ranging from 3 to 18. The observed and expected heterozygosities ranged from 0.3 to 1 and from 0.426 to 0.93, respectively. Only one tested locus significantly deviated from Hardy-Weinberg equilibrium. 18 microsatellite loci were highly polymorphic (PIC > 0.5). These newly isolated microsatellite markers would be useful to study the population genetics and stock management ofX. davidi.

引用

页码：8305 / 8310

页数：6

共 27 条

[1] Cross-species transfer of nuclear microsatellite markers:: potential and limitations [J].