Metabolic Engineering-Based Rapid Characterization of a Sesquiterpene Cyclase and the Skeletons of Fusariumdiene and Fusagramineol from Fusarium graminearum

被引：31

作者：

Bian, Guangkai ^{[1
,2
,5
]}

Hou, Anwei ^{[1
,2
,5
]}

Yuan, Yujie ^{[1
,2
]}

Hu, Ben ^{[1
,2
]}

Cheng, Shu ^{[1
,2
]}

Ye, Ziling ^{[2
,3
]}

Di, Yingtong ^{[4
,5
]}

Deng, Zixin ^{[1
,2
,5
]}

Liu, Tiangang ^{[1
,2
,5
]}

机构：

[1] Wuhan Univ, Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Hubei, Peoples R China

[2] Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Hubei, Peoples R China

[3] J1 Biotech Co Ltd, Wuhan 430075, Hubei, Peoples R China

[4] Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China

[5] Wuhan Inst Biotechnol, Hubei Engn Lab Synthet Microbiol, Wuhan 430075, Hubei, Peoples R China

来源：

ORGANIC LETTERS | 2018年 / 20卷 / 06期

基金：

中国博士后科学基金;

关键词：

PLANT-PATHOGENIC FUNGUS; BIOSYNTHESIS; SYNTHASE; OVERPRODUCTION; TRICHODIENE; CYCLIZATION; MECHANISM; FUJIKUROI;

D O I：

10.1021/acs.orglett.8b00366

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The potential power of sesquiterpene synthase FgJ03939 from Fusarium graminearum was fully exploited in a farnesyl diphosphate-overexpressing Saccharomyces cerevisiae chassis to produce the novel sesquiterpenes fusariumdiene (1), epi-fusagramineol (2), and fusagramineol (3) with 5/7 bicyclic and 5/6/3 tricyclic ring systems, respectively, as well as five known sesquiterpenes (4-8). The structure of the unusual skeletons was characterized, and an absolute configuration was proposed. A mechanism for the biosynthesis of 1-8 was also proposed.

引用

页码：1626 / 1629

页数：4