Detection and partial molecular characterization of Grapevine fleck virus, Grapevine virus D, Grapevine leafroll-associated virus -5 and-6 infecting grapevines in Brazil

被引：2

作者：

Martins Fajardo, Thor Vinicius ^{[1
]}

Eiras, Marcelo ^{[2
]}

Nickel, Osmar ^{[1
]}

Dubiela, Carla Rosa ^{[3
]}

de Souto, Eliezer Rodrigues ^{[3
]}

机构：

[1] Embrapa Uva & Vinho, BR-95700000 Bento Goncalves, RS, Brazil

[2] Inst Biol Sao Paulo, Sao Paulo, Brazil

[3] Univ Estadual Maringa, Dept Agron, Maringa, Parana, Brazil

来源：

CIENCIA RURAL | 2012年 / 42卷 / 12期

关键词：

diagnosis; sequencing; Maculavirus; Vitivirus; Ampelovirus; Vitis; GENOME ORGANIZATION;

D O I：

10.1590/S0103-84782012005000119

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Grapevine fleck, rugose wood and leafroll are three grapevine viral diseases whose causal agents (or associated viruses) respectively are Grapevine fleck virus (GFkV), Grapevine virus D (GVD) and Grapevine leafroll-associated virus 5 and 6 (GLRaV-5 and -6). The objective of this work was to perform a partial molecular characterization of local isolates of these four viral species that infect grapevines. The nucleotide and deduced amino acid sequences of complete genes of the coat protein (CP) (of GFkV), the CP and the RNA binding protein (of GVD), the CP and the partial hHSP70 gene (of GLRaV-5) and the partial hHSP70 gene (of GLRaV-6) were aligned and compared in silico with other isolates. These data extend the available information about Brazilian isolates of GFkV, GLRaV-5 and -6, and reports for the first time the GVD occurrence in Brazil.

引用

页码：2127 / 2130

页数：4

共 12 条

[11] Complete nucleotide sequence and genome organization of Grapevine fleck virus
Sabanadzovic, S
Abou Ghanem-Sabanadzovic, N
Saldarelli, P
Martelli, GP
[J]. JOURNAL OF GENERAL VIROLOGY, 2001, 82 : 2009 - 2015
[12] Genomic analysis of Grapevine leafroll associated virus-5 and related viruses
Thompson, Jeremy R.
Fuchs, Marc
Perry, Keith L.
[J]. VIRUS RESEARCH, 2012, 163 (01) : 19 - 27

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