Reassessment of the PAS patterns in uveal melanoma

被引:51
作者
Foss, AJE
Alexander, RA
Hungerford, JL
Harris, AL
Lightman, S
机构
[1] INST OPHTHALMOL,DEPT CLIN SCI,LONDON EC1V 9EL,ENGLAND
[2] INST OPHTHALMOL,DEPT PATHOL,LONDON EC1V 9EL,ENGLAND
[3] MOORFIELDS EYE HOSP,ONCOL CLIN,LONDON EC1V 2PD,ENGLAND
[4] CHURCHILL HOSP,DEPT ONCOL,OXFORD OX3 7LJ,ENGLAND
关键词
D O I
10.1136/bjo.81.3.240
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Background-Previous work has highlighted the prognostic importance of patterns of periodic acid Schiff (PAS) staining (the Folberg patterns) in uveal melanoma. These patterns have been ascribed to blood vessels but the patterns are different from those seen with other staining techniques for blood vessels. It has recently been shown that microvessel density is the dominant prognostic factor in uveal melanoma. This study reinvestigates the nature and significance of the PAS patterns. Methods-The PAS patterns were compared with the patterns seen with conventional connective tissue stains and with the patterns seen in sections stained for the presence of blood vessels (by immunohistochemistry for factor VIII related antigen). The PAS patterns were determined on a panel of 117 cases of uveal melanoma. The prognostic significance of each of these patterns was determined and, as more than one pattern can exist in a tumour, principal components analysis was performed to determine the number of underlying factors. Results-Comparison of the PAS patterns with other stains demonstrates that they are based on connective tissue including fibrovascular tissue. Five of the nine PAS patterns carried prognostic significance on univariate analysis. Principal components analysis suggested that these patterns represented three underlying factors, which were tentatively identified as representing disordered growth (factor 1), emergence of rapidly growing subclones (factor 2), and section orientation (factor 3). Conclusions-The PAS patterns are based on fibrovascular tissue and can be ascribed to three underlying factors. The first two of these factors carried prognostic significance and the first (disordered growth) retained independent prognostic significance in a multivariate Cox model which included microvessel density and tumour size.
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页码:240 / 246
页数:9
相关论文
共 29 条
[1]   ELASTIC AND PRECURSOR FIBERS IN THE NORMAL HUMAN-EYE [J].
ALEXANDER, RA ;
GARNER, A .
EXPERIMENTAL EYE RESEARCH, 1983, 36 (02) :305-315
[2]  
ALEXANDER RA, 1986, MED LAB SCI, V43, P121
[3]  
BAINS RM, 1988, MED LAB SCI, V45, P240
[4]   MICROVESSEL QUANTITATION AND PROGNOSIS IN INVASIVE BREAST-CARCINOMA [J].
BOSARI, S ;
LEE, AKC ;
DELELLIS, RA ;
WILEY, BD ;
HEATLEY, GJ ;
SILVERMAN, ML .
HUMAN PATHOLOGY, 1992, 23 (07) :755-761
[5]  
CULLING CFA, 1974, HDB HISTOPATHOLOGICA, P609
[6]  
FOLBERG R, 1993, OPHTHALMOLOGY, V100, P1389
[7]   THE MORPHOLOGIC CHARACTERISTICS OF TUMOR BLOOD-VESSELS AS A MARKER OF TUMOR PROGRESSION IN PRIMARY HUMAN UVEAL MELANOMA - A MATCHED CASE-CONTROL STUDY [J].
FOLBERG, R ;
PEER, J ;
GRUMAN, LM ;
WOOLSON, RF ;
JENG, G ;
MONTAGUE, PR ;
MONINGER, TO ;
YI, H ;
MOORE, KC .
HUMAN PATHOLOGY, 1992, 23 (11) :1298-1305
[8]  
Foss AJE, 1996, CANCER RES, V56, P2900
[9]  
FOSS AJE, 1995, BRIT J BIOMED SCI, V52, P22
[10]  
Gomori G, 1937, AM J PATHOL, V13, P993