Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis

被引：643

作者：

Stroud, Hume ^{[1
]}

Do, Truman ^{[1
]}

Du, Jiamu ^{[2
]}

Zhong, Xuehua ^{[1
]}

Feng, Suhua ^{[1
,3
,4
]}

Johnson, Lianna ^{[1
]}

Patel, Dinshaw J. ^{[2
]}

Jacobsen, Steven E. ^{[1
,3
,4
]}

机构：

[1] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90047 USA

[2] Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY 10021 USA

[3] Univ Calif Los Angeles, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA USA

[4] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90024 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2014年 / 21卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

DIRECTED DNA-METHYLATION; H3; LYSINE-9; METHYLATION; RNA-POLYMERASE-IV; HISTONE METHYLATION; CPG METHYLATION; POL IV; METHYLTRANSFERASE; HETEROCHROMATIN; GENOME; DIFFERENTIATION;

D O I：

10.1038/nsmb.2735

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA methylation occurs in CG and non-CG sequence contexts. Non-CG methylation is abundant in plants and is mediated by CHROMOMETHYLASE (CMT) and DOMAINS REARRANGED METHYLTRANSFERASE (DRM) proteins; however, its roles remain poorly understood. Here we characterize the roles of non-CG methylation in Arabidopsis thaliana. We show that a poorly characterized methyltransferase, CMT2, is a functional methyltransferase in vitro and in vivo. CMT2 preferentially binds histone H3 Lys9 (H3K9) dimethylation and methylates non-CG cytosines that are regulated by H3K9 methylation. We revealed the contributions and redundancies between each non-CG methyltransferase in DNA methylation patterning and in regulating transcription. We also demonstrate extensive dependencies of small-RNA accumulation and H3K9 methylation patterning on non-CG methylation, suggesting self-reinforcing mechanisms between these epigenetic factors. The results suggest that non-CG methylation patterns are critical in shaping the landscapes of histone modification and small noncoding RNA.

引用

页码：64 / +

页数：10

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