Mycosynthesis of bactericidal silver and polymorphic gold nanoparticles: physicochemical variation effects and mechanism

被引：18

作者：

Singh, Dheeraj Kumar ^{[1
]}

Kumar, Jitendra ^{[1
]}

Sharma, Vijay Kumar ^{[1
]}

Verma, Satish Kumar ^{[1
]}

Singh, Arti ^{[1
]}

Kumari, Puja ^{[1
]}

Kharwar, Ravindra Nath ^{[1
]}

机构：

[1] Banaras Hindu Univ, Inst Sci, Dept Bot, Mycopathol & Microbial Technol Lab, Varanasi 221005, Uttar Pradesh, India

来源：

NANOMEDICINE | 2018年 / 13卷 / 02期

关键词：

biosynthesis mechanism; metal nanoparticles; mycofabrication; ANTIMICROBIAL ACTIVITY; MEDICAL APPLICATIONS; METAL NANOPARTICLES; MEDIATED SYNTHESIS; CYTOCHROME-C; BIOSYNTHESIS; REDUCTION; PEPTIDES; VIRUS; IONS;

D O I：

10.2217/nnm-2017-0235

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: Extracellular synthesis of silver and gold nanoparticles using aqueous cell-free filtrate (CFF) of endophytic Chaetomium globosum and characterization of its bioactive proteins. Methods: Temperature and pH gradients were used to assess their effects on dimensions of NPs. NPs were tested in vivo for antibacterial activity. MALDI-TOF-MS/MS was used for characterization of CFF proteins. Results: Fungal CFF fabricated nanoparticles of various shape under varied physicochemical conditions. Silver nanoparticles showed significantly (p <= 0.5) enhanced antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae compared with AgNO3. Two prominent CFF proteins showed homology with benzoate 4-monooxygenase cytochrome P450 and ubiquinol-cytochrome c reductase. Conclusion: The study achieved controlled mycosynthesis of NPs and explains the hitherto poorly known mechanism of reduction, stabilization and antibacterial activity of nanoparticles.

引用

页码：191 / 207

页数：17

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