Development Perspective of Bioelectrocatalysis-Based Biosensors

被引：30

作者：

Adachi, Taiki ^{[1
]}

Kitazumi, Yuki ^{[1
]}

Shirai, Osamu ^{[1
]}

Kano, Kenji ^{[1
,2
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan

[2] Kyoto Univ, Ctr Adv Sci & Innovat, Uji, Kyoto 6110011, Japan

来源：

SENSORS | 2020年 / 20卷 / 17期

基金：

日本学术振兴会;

关键词：

current-potential curve; multi-enzymatic cascades; multianalyte detection; mass-transfer-controlled amperometric response; potentiometric coulometry; DIRECT ELECTRON-TRANSFER; AMPEROMETRIC BIOSENSOR; GLUCOSE BIOSENSORS; ELECTROCHEMICAL BIOSENSORS; CHRONOAMPEROMETRIC CURRENT; CHOLESTEROL ESTERASE; BAND MICROELECTRODES; ENZYME ELECTRODES; CARBON NANOTUBES; ALCOHOL OXIDASE;

D O I：

10.3390/s20174826

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.

引用

页码：1 / 21

页数：21

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