Self-calibrating viscosity probes: Design and subcellular localization

被引：23

作者：

Dakanali, Marianna ^{[1
]}

Do, Thai H. ^{[1
]}

Horn, Austin ^{[2
]}

Chongchivivat, Akaraphon ^{[1
]}

Jarusreni, Tuptim ^{[1
]}

Lichlyter, Darcy ^{[2
]}

Guizzunti, Gianni ^{[3
]}

Haidekker, Mark A. ^{[2
]}

Theodorakis, Emmanuel A. ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[2] Univ Georgia, Fac Engn, Athens, GA 30602 USA

[3] Inst Pasteur, Dept Cell Biol & Infect, Membrane Traff & Pathogenesis Unit, Paris, France

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2012年 / 20卷 / 14期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Fluorescence; Ratiometric dye; Molecular rotor; Subcellular viscosity; FLUORESCENCE PHOTOBLEACHING RECOVERY; MOLECULAR ROTOR; BLOOD-VISCOSITY; DIFFUSION; BINDING; RHEOLOGY; FLUIDITY; SENSOR; CELLS;

D O I：

10.1016/j.bmc.2012.05.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the design, synthesis and fluorescence profiles of new self-calibrating viscosity dyes in which a coumarin (reference fluorophore) has been covalently linked with a molecular rotor (viscosity sensor). Characterization of their fluorescence properties was made with separate excitation of the units and through resonance energy transfer from the reference to the sensor dye. We have modified the linker and the substitution of the rotor in order to change the hydrophilicity of these probes thereby altering their subcellular localization. For instance, hydrophilic dye 12 shows a homogeneous distribution inside the cell and represents a suitable probe for viscosity measurements in the cytoplasm. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：4443 / 4450

页数：8

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