Evidence for a novel biological role for the multifunctional β-1,3-glucan binding protein in shrimp

被引：32

作者：

Goncalves, Priscila ^{[1
]}

Vernal, Javier ^{[2
]}

Rosa, Rafael Diego ^{[3
,4
]}

Yepiz-Plascencia, Gloria ^{[5
]}

Batista de Souza, Claudia Regina ^{[6
]}

Barracco, Margherita Anna ^{[1
]}

Perazzolo, Luciane Maria ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, Ctr Ciencias Biol, Dept Biol Celular Embriol & Genet, Lab Imunol Aplicada Aquicultura, BR-88040900 Florianopolis, SC, Brazil

[2] Univ Fed Santa Catarina, Dept Bioquim, Ctr Biol Mol Estrutural, BR-88040900 Florianopolis, SC, Brazil

[3] Univ Montpellier I, Univ Montpellier 2, CNRS, Ifremer, F-34095 Montpellier, France

[4] IRD, UMR Ecol Syst Marins Cotiers 5119, F-34095 Montpellier, France

[5] Ctr Invest Alimentaci & Desarrollo AC, Hermosillo 83000, Sonora, Mexico

[6] Fed Univ Para, Inst Ciencias Biol, Mol Biol Lab, BR-66075110 Belem, Para, Brazil

来源：

MOLECULAR IMMUNOLOGY | 2012年 / 51卷 / 3-4期

关键词：

Pattern recognition protein/receptor; beta GBP; Shrimp immunity; Prophenoloxidase activating system; Lectin; Transcriptional response to fungal infection; PATTERN-RECOGNITION PROTEIN; GENE-EXPRESSION; LITOPENAEUS-VANNAMEI; SYSTEM; PURIFICATION; LIPOPROTEIN; ACTIVATION; HEMOLYMPH; IMMUNITY; CLONING;

D O I：

10.1016/j.molimm.2012.03.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-1,3-Glucan binding proteins (beta GBPs) are soluble pattern recognition proteins/receptors that bind to beta-1,3-glucans from fungi cell walls. In crustaceans, beta GBPs are abundant plasmatic proteins produced by the hepatopancreas, and have been proved to play multiple biological functions. Here, we purified and characterized novel members of the beta GBP family from the hemolymph of two Brazilian shrimps, Farfantepenaeus paulensis (Fp beta GBP) and Litopenaeus schmitti (Ls beta GBP). As observed for other crustacean species, Fp beta GBP and Ls beta GBP are monomeric proteins (similar to 100 kDa) able to enhance the activation of the prophenoloxidase system, a potent antimicrobial defense conserved in arthropods. More interestingly, we provided here evidence for a novel biological activity for shrimp beta GBPs: the agglutination of fungal cells. Finally, we investigated the modulation of the beta GBP gene in F. paulensis shrimps experimentally infected with a cognate fungal pathogen, Fusarium solani. From our expression data, beta GBP gene is constitutively expressed in hepatopancreas and not modulated upon a non-lethal fungal infection. Herein, we have improved our knowledge about the beta GBP family by the characterization of a novel biological role for this multifunctional protein in shrimp. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：363 / 367

页数：5

共 33 条

[1] THE BETA-1,3-GLUCAN-BINDING PROTEIN FROM THE CRAYFISH PACIFASTACUS-LENIUSCULUS, WHEN REACTED WITH A BETA-1,3-GLUCAN, INDUCES SPREADING AND DEGRANULATION OF CRAYFISH GRANULAR CELLS [J].