Untargeted foodomics strategy using high-resolution mass spectrometry reveals potential indicators for fish freshness

被引:38
作者
Chang, William Chih-Wei [1 ]
Wu, Hsin-Yi [2 ]
Yeh, Yun [3 ]
Liao, Pao-Chi [1 ,3 ]
机构
[1] Natl Cheng Kung Univ, Coll Med, Dept Environm & Occupat Hlth, Tainan 704, Taiwan
[2] Natl Taiwan Univ, Instrumentat Ctr, Taipei 106, Taiwan
[3] Natl Cheng Kung Univ, Coll Med, Dept Food Safety Hyg & Risk Management, Tainan 701, Taiwan
关键词
Metabolomics; Food safety; Biomarker discovery; Spoilage; Storage; Tuna; SALMON SALMO-SALAR; COD GADUS-MORHUA; FATTY-ACIDS; NUCLEOTIDE DEGRADATION; METABOLOMICS ANALYSIS; POSTMORTEM CHANGES; LIPID HYDROLYSIS; WATER FISH; HYPOXANTHINE; STORAGE;
D O I
10.1016/j.aca.2020.06.016
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Fish among other food can easily become spoilage. However, the existing literature endeavouring into a holistic investigation of fish metabolome during storage is scarce. There is a need for a powerful tool for more in-depth determinations. The present study is leveraging high-resolution mass spectrometry (HRMS)-based untargeted foodomics in the exploration of potential indicators for fish freshness. Three batches of fish fillets were stored in the refrigerator (+4 degrees C) for 0, 24, 48, and 72 h. Features were detected via UPLC-Q-Orbitrap HRMS and hereby undergone selection, identification, and verification. Eight sensitive indicators with significant time-dependent responses were yielded. The loss of freshness in fish is manifested in the upregulation of uracil, hypoxanthine, and inosine (involved in nucleotide changes) and alpha-linolenic acid, docosahexaenoic acid, arachidonic acid, and linoleic acid (involved in lipid hydrolysis) as well as in the downregulation of decanoylcarnitine (involved in fatty acid metabolism). Our work provides a promising approach to assess the quality of fish during storage and gain deeper insights into the metabolic reaction. (C) 2020 Elsevier B.V. All rights reserved.
引用
收藏
页码:98 / 105
页数:8
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