B subunit of Escherichia coli heat-labile enterotoxin as adjuvant of humoral immune response in recombinant BCG vaccination

被引：0
作者：
Ramos Rocha, Andrea da Silva [1 ]
Conceicao, Fabricio Rochedo [1 ,2 ]
Grassmann, Andre Alex [1 ]
Lagranha, Valeska Lizzi [1 ]
Dellagostin, Odir Antonio [1 ,3 ]
机构：
[1] Univ Fed Pelotas, Ctr Biotecnol, BR-96010900 Pelotas, RS, Brazil
[2] Univ Fed Pelotas, Fac Vet, BR-96010900 Pelotas, RS, Brazil
[3] Univ Fed Pelotas, Inst Biol, BR-96010900 Pelotas, RS, Brazil
来源：
CANADIAN JOURNAL OF MICROBIOLOGY | 2008年 / 54卷 / 08期
关键词：
LTB; adjuvant; recombinant BCG; humoral immune response;
D O I：
10.1139/W08-056
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to recombinant BCG (rBCG) vaccination was evaluated. Isogenic mice were immunized with rBCG expressing the R1 repeat region of the P97 adhesin of Mycoplasma hyopneumoniae alone (rBCG/R1) or fused LTB (rBCG/LTBR1). Anti-R1 systemic antibody levels (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA) were measured by ELISA using recombinant R1 as antigen. With the exception of IgM. LTB doubled the anti-R1 antibody levels in rBCG vaccination. The IgG1/IgG2a mean ratio showed that both rBCG/LTBR1 and rBCG/R1 induced a mixed Th1/Th2 immune response. Interestingly, anti-R1 serum IgA was induced only by rBCG/LTBR1. These results demonstrate that LTB has an adjuvant effect on the humoral immune response to recombinant antigens expressed in BCG.
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页码：677 / 686
页数：10
相关论文
共 45 条
[1]  
[Anonymous], 2001, Anal Biochem
[2]   Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a truncated form of GP5 and M protein of porcine reproductive and respiratory syndrome virus [J].
Bastos, RG ;
Dellagostin, OA ;
Barletta, RG ;
Doster, AR ;
Nelson, E ;
Zuckermann, F ;
Osorio, FA .
VACCINE, 2004, 22 (3-4) :467-474
[3]   Immune response induced by recombinant Mycobacterium bovis BCG producing the cholera toxin B subunit [J].
Biet, F ;
Kremer, L ;
Wolowczuk, I ;
Delacre, M ;
Locht, C .
INFECTION AND IMMUNITY, 2003, 71 (05) :2933-2937
[4]   Bacterial and plant enterotoxin B subunit-autoantigen fusion proteins suppress diabetes insulitis [J].
Carter, JE ;
Yu, F ;
Choi, NW ;
Hough, J ;
Henderson, D ;
He, D ;
Langridge, WHR .
MOLECULAR BIOTECHNOLOGY, 2006, 32 (01) :1-15
[5]   Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopneumoniae as a mucosal vaccine in mice [J].
Chen, Austen Y. ;
Fry, Scott R. ;
Forbes-Faulkner, Judy ;
Daggard, Grant ;
Mukkur, T. K. S. .
JOURNAL OF MEDICAL MICROBIOLOGY, 2006, 55 (07) :923-929
[6]   A recombinant chimera composed of repeat region RR1 of Mycoplasma hyopneumoniae adhesin with Pseudomonas exotoxin:: in vivo evaluation of specific IgG response in mice and pigs [J].
Chen, JR ;
Liao, CW ;
Mao, SJT ;
Weng, CN .
VETERINARY MICROBIOLOGY, 2001, 80 (04) :347-357
[7]  
CRUZ FW, 2001, VACCINE, V20, P731, DOI DOI 10.1016/SO264-410X(01)00414-5
[8]   Role of GM1 binding in the mucosal immunogenicity and adjuvant activity of the Escherichia coli heat-labile enterotoxin and its B subunit [J].
De Haan, L ;
Verweij, WR ;
Feil, IK ;
Holtrop, M ;
Hol, WGJ ;
Agsteribbe, E ;
Wilschut, J .
IMMUNOLOGY, 1998, 94 (03) :424-430
[9]   ACTIVITY OF MYCOBACTERIAL PROMOTERS DURING INTRACELLULAR AND EXTRACELLULAR GROWTH [J].
DELLAGOSTIN, OA ;
ESPOSITO, G ;
EALES, LJ ;
DALE, JW ;
MCFADDEN, J .
MICROBIOLOGY-UK, 1995, 141 :1785-1792
[10]   Optimized immune response elicited by a DNA vaccine expressing pneumococcal surface protein a is characterized by a balanced immunoglobulin G1 (IgG1)/IgG2a ratio and proinflammatory cytokine production [J].
Ferreira, Daniela M. ;
Darrieux, Michelle ;
Oliveira, Maria Leonor S. ;
Leite, Luciana C. C. ;
Miyaji, Eliane N. .
CLINICAL AND VACCINE IMMUNOLOGY, 2008, 15 (03) :499-505
12345