Soluble Expression of OmpA from Haemophilus parasuis in Escherichia coli and Its Protective Effects in the Mouse Model of Infection

被引：6

作者：

Ahn, Jungoh ^{[1
]}

Hong, Minhee ^{[1
]}

Yoo, Sungsik ^{[2
]}

Lee, Eungyo ^{[1
]}

Won, Hokeun ^{[2
]}

Yoon, Injoong ^{[2
]}

Jung, Joon-ki ^{[1
]}

Lee, Hongweon ^{[1
]}

机构：

[1] KRIBB, Biotechnol Proc Engn Ctr, Taejon 305600, South Korea

[2] Choong Ang Vaccine Lab, Taejon 305348, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2012年 / 22卷 / 09期

关键词：

Haemophilus parasuis; soluble expression; pelB leader sequence; antigen; OmpA; Escherichia coli; OUTER-MEMBRANE PROTEINS; CROSS-PROTECTION; SEROVAR; 5; PIGS; STRAINS;

D O I：

10.4014/jmb.1201.01003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Haemophilus parasols causes contagious porcine Glasser's disease leading to severe losses in the swine industry. In this study, we established an efficient Escherichia coli-based system for the expression of H. parasols major outer-membrane protein (MOMP) that has been known as a good vaccine candidate against Glasser's disease. Use of an E. coli-derived pelB leader sequence made it possible to produce recombinant MOMP (rMOMP) as the soluble forms without an additional refolding process. Using two different animal models, it was evaluated that the rMOMP was capable of inducing a significant immune response and providing protection against H. parasols infection.

引用

页码：1307 / 1309

页数：3

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