Porous Polysaccharide-Based Scaffolds for Human Endothelial Progenitor Cells

被引：41

作者：

Lavergne, Melanie ^{[1
,2
,3
]}

Derkaoui, Mohammed ^{[4
]}

Delmau, Catherine ^{[2
]}

Letourneur, Didier ^{[1
,4
]}

Uzan, Georges ^{[2
]}

Le Visage, Catherine ^{[1
,4
]}

机构：

[1] Univ Paris 13, Sorbonne Paris Cite,Inst Galilee, Lab Bioingn Polymeres Cardiovasc BPC, F-75025 Paris, France

[2] Hop Paul Brousse, INSERM, U972, F-94807 Villejuif, France

[3] ABCell Bio SAS, F-75009 Paris, France

[4] CHU Xavier Bichat, INSERM, U698, F-75877 Paris 18, France

来源：

MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 07期

关键词：

biomaterials; endothelial progenitor cells; hydrogels; polysaccharides; porous scaffolds; UMBILICAL-CORD BLOOD; ANGIOGENESIS; DELIVERY; ANOIKIS; LINKING;

D O I：

10.1002/mabi.201100431

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human ECFCs contribute to vascular repair. For this reason, they are considered as valuable cell therapy products in ischemic diseases. Porous scaffolds are prepared that are composed of natural polysaccharides, pullulan and dextran, by chemical crosslinking without use of organic solvents. These porous scaffolds, which have pores with an average size of 42?mu m and a porosity of 21%, preserve the viability and the proliferation of cord-blood ECFCs. After 7 d of culture in porous scaffolds, ECFCs express endothelial markers (CD31 and vWf) and maintain endothelial functions. The cultured cells can be easily retrieved by enzymatic degradation of the porous scaffolds. In vitro results suggest that the porous scaffold could allow cell delivery of ECFCs for treatment of vascular diseases.

引用

页码：901 / 910

页数：10

共 33 条

[1] Influence of polysaccharide composition on the biocompatibility of pullulan/dextran-based hydrogels [J].

Abed, Aicha ;

Assoul, Nabila ;

Ba, Maguette ;

Derkaoui, Sidi Mohamed ;

Portes, Patrick ;

Louedec, Liliane ;

Flaud, Patrice ;

Bataille, Isabelle ;

Letourneur, Didier ;

Meddahi-Pelle, Anne .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2011, 96A (03) :535-542

[2] Monocyte activation in angiogenesis and collateral growth in the rabbit hindlimb [J].

Arras, M ;

Ito, WD ;

Scholz, D ;

Winkler, B ;

Schaper, J ;

Schaper, W .

JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (01) :40-50

[3] Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels [J].

Au, Patrick ;

Daheron, Laurence M. ;

Duda, Dan G. ;

Cohen, Kenneth S. ;

Tyrrell, James A. ;

Lanning, Ryan M. ;

Fukumura, Dai ;

Scadden, David T. ;

Jain, Rakesh K. .

BLOOD, 2008, 111 (03) :1302-1305

[4] Pullulan-based hydrogel for smooth muscle cell culture [J].

Autissier, Aude ;

Letourneur, Didier ;

Le Visage, Catherine .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 82A (02) :336-342

[5] Fabrication of porous polysaccharide-based scaffolds using a combined freeze-drying/cross-linking process [J].

Autissier, Aude ;

Le Visage, Catherine ;

Pouzet, Cecile ;

Chaubet, Frederic ;

Letourneur, Didier .

ACTA BIOMATERIALIA, 2010, 6 (09) :3640-3648

[6] Human endothelial cells derived from circulating progenitors display specific functional properties compared with mature vessel wall endothelial cells [J].

Bompais, H ;

Chagraoui, J ;

Canron, X ;

Crisan, M ;

Liu, XH ;

Anjo, A ;

Port, CTL ;

Leboeuf, M ;

Charbord, P ;

Bikfalvi, A ;

Uzan, G .

BLOOD, 2004, 103 (07) :2577-2584

[7] Angiogenesis in health and disease [J].

Carmeliet, P .

NATURE MEDICINE, 2003, 9 (06) :653-660

[8]

Carpentier G., 2007, IMAGE J MACRO

[9] The evaluation of a small-diameter polysaccharide-based arterial graft in rats [J].

Chaouat, Marc ;

Le Visage, Catherine ;

Autissier, Aude ;

Chaubet, Frederic ;

Letourneur, Didier .

BIOMATERIALS, 2006, 27 (32) :5546-5553

[10] Endothelial colony-forming cell role in neoangiogenesis and tissue repair [J].

Critser, Paul J. ;

Yoder, Mervin C. .

CURRENT OPINION IN ORGAN TRANSPLANTATION, 2010, 15 (01) :68-72

← 1 2 3 4 →