REDUCED RESPONSIVENESS OF BLOOD LEUKOCYTES TO LIPOPOLYSACCHARIDE DOES NOT PREDICT NOSOCOMIAL INFECTIONS IN CRITICALLY ILL PATIENTS

被引:17
作者
van Vught, Lonneke A. [1 ]
Wiewel, Maryse A. [1 ]
Hoogendijk, Arie J. [1 ]
Sciciuna, Brendon P. [1 ]
Belkasim-Bohoudi, Hakima [1 ]
Horn, Janneke [2 ]
Schultz, Marcus J. [2 ]
van der Poll, Tom [1 ,3 ]
机构
[1] Univ Amsterdam, Acad Med Ctr, Ctr Expt & Mol Med, NL-1105 AZ Amsterdam, Netherlands
[2] Univ Amsterdam, Acad Med Ctr, Dept Intens Care, NL-1105 AZ Amsterdam, Netherlands
[3] Univ Amsterdam, Acad Med Ctr, Div Infect Dis, NL-1105 AZ Amsterdam, Netherlands
来源
SHOCK | 2015年 / 44卷 / 02期
关键词
Nosocomial infection; whole-blood stimulation; critically ill; intensive care unit; immunosuppression; IMMUNE DYSFUNCTIONS; SEPSIS; DEFINITIONS;
D O I
10.1097/SHK.0000000000000391
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Critically ill patients show signs of immune suppression, which is considered to increase vulnerability to nosocomial infections. Whole-blood stimulation is frequently used to test the function of the innate immune system. We here assessed the association between whole-blood leukocyte responsiveness to lipopolysaccharide (LPS) and subsequent occurrence of nosocomial infections in critically ill patients admitted to the intensive care unit (ICU). All consecutive critically ill patients admitted to the ICU between April 2012 and June 2013 with two or more systemic inflammatory response syndrome criteria and an expected length of ICU stay of more than 24 h were enrolled. Age- and sex-matched healthy individuals were included as controls. Blood was drawn the first morning after ICU admission and stimulated ex vivo with 100 ng/mL ultrapure LPS for 3 h. Tumor necrosis factor-, interleukin-1 (IL-1), and IL-6 were measured in supernatants. Seventy-three critically ill patients were included, of whom 10 developed an ICU-acquired infection. Compared with healthy subjects, whole-blood leukocytes of patients were less responsive to ex vivo stimulation with LPS, as reflected by strongly reduced tumor necrosis factor-, IL-1, and IL-6 levels in culture supernatants. Results were not different between patients who did and those who did not develop an ICU-acquired infection. The extent of reduced LPS responsiveness of blood leukocytes in critically ill patients on the first day after ICU admission does not relate to the subsequent development of ICU-acquired infections. These results argue against the use of whole-blood stimulation as a functional test applied early after ICU admission to predict nosocomial infection.
引用
收藏
页码:110 / 114
页数:5
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