Sensitive detection of alkaline phosphatase by dephosphorylation-initiated transcription reaction-mediated dual signal amplification

被引：61

作者：

Ma, Fei ^{[1
]}

Liu, Wen-jing ^{[1
]}

Liang, Li ^{[2
]}

Tang, Bo ^{[1
]}

Zhang, Chun-Yang ^{[1
]}

机构：

[1] Shandong Normal Univ, Shandong Prov Key Lab Clean Prod Fine Chem, Key Lab Mol & Nano Probes,Minist Educ, Coll Chem Chem Engn & Mat Sci,Collaborat Innovat, Jinan 250014, Shandong, Peoples R China

[2] Peking Univ, Hosp 3, Dept Tumor Chemotherapy & Radiat Sickness, Beijing 100191, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 19期

基金：

中国国家自然科学基金;

关键词：

DUPLEX-SPECIFIC NUCLEASE; TIME FLUORESCENCE ASSAY; PERYLENE-PROBE; LIVING CELLS; COPPER NANOPARTICLES; LAMBDA EXONUCLEASE; RNA-POLYMERASE; QUANTUM DOTS; STRATEGY; DNA;

D O I：

10.1039/c8cc00540k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We develop a new fluorescence method for the sensitive detection of alkaline phosphatase (ALP) based on dephosphorylation-initiated transcription reaction-mediated dual signal amplification. This research demonstrates for the first time a significant improvement in ALP assay performance by using a nucleic acid amplification strategy, which enables sensitive detection of ALP activity in vitro and in cell extracts. Importantly, this method can be further applied for kinetic analysis and ALP inhibition assay.

引用

页码：2413 / 2416

页数：4

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