Reflectance confocal microscopy of red blood cells: simulation and experiment

被引：15

作者：

Zeidan, Adel ^{[1
]}

Yelin, Dvir ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Fac Biomed Engn, IL-3200003 Haifa, Israel

来源：

BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 11期

基金：

以色列科学基金会;

关键词：

DIGITAL HOLOGRAPHIC MICROSCOPY; SCANNING LASER MICROSCOPY; ENCODED FLOW-CYTOMETRY; IN-VIVO; HUMAN SKIN; MORPHOLOGY;

D O I：

10.1364/BOE.6.004335

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Measuring the morphology of red blood cells is important for clinical diagnosis, providing valuable indications on a patient's health. In this work, we have simulated the appearance of normal red blood cells under a reflectance confocal microscope and discovered unique relations between the morphological parameters and the resulting characteristic interference patterns of the cell. The simulation results showed good agreement with in vitro reflectance confocal images of red blood cells, acquired using spectrally encoded flow cytometry that imaged the cells in a linear flow without artificial staining. By matching the simulated patterns to confocal images of the cells, this method could be used for measuring cell morphology in three dimensions and for studying their physiology. (C) 2015 Optical Society of America

引用

页码：4335 / 4343

页数：9

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