A fluorine-doped tin oxide electrode modified with gold nanoparticles for electrochemiluminescent determination of hydrogen peroxide released by living cells

被引：29

作者：

Li, Man ^{[1
]}

Gao, Hongfang ^{[1
]}

Wang, Xiaofei ^{[1
]}

Wang, Yufeng ^{[1
]}

Qi, Honglan ^{[1
]}

Zhang, Chengxiao ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China

来源：

MICROCHIMICA ACTA | 2017年 / 184卷 / 02期

关键词：

ECL; FTO; Gold nanoparticles; Luminol; Hydrogen peroxide; Macrophage cells; HeLa cells; ELECTROGENERATED CHEMILUMINESCENCE; LUMINOL ELECTROCHEMILUMINESCENCE; FLUORESCENT-PROBE; ALKALINE-SOLUTION; QUANTUM DOTS; GLUCOSE; VISUALIZATION; ENHANCEMENT; NANOTUBES; BIOSENSOR;

D O I：

10.1007/s00604-016-2051-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrogenerated chemiluminescence (ECL) based method is described for the determination of hydrogen peroxide (H2O2) released by living cells. It is making use of a fluorine-doped tin oxide (FTO) electrode that was modified with gold nanoparticles (AuNPs). The AuNP-FTO was fabricated by electrodeposition of AuNP on the surface of the FTO electrode and characterized by scanning electron microscopy and electrochemical techniques. The AuNP-FTO exhibits a significant electrocatalytic activity towards luminol-based ECL system compared to a bare FTO. The calibration plot covers the 10 nM to 1 mu M H2O2 concentration range, with a 8 nM detection limit (at a signal-to-noise ratio of 3). The method enabled (a) the monitoring of drug-stimulated production of H2O2 released by living cells including RAW 264.7 macrophage cells and HeLa cells; and (b) the determination of inhibitors of H2O2 secretion. The method, due to strong signal amplification by AuNPs, provides a viable tool for determination of H2O2 in biological systems.

引用

页码：603 / 610

页数：8

共 34 条

[1] MONITORING AN OXIDATIVE STRESS MECHANISM AT A SINGLE HUMAN FIBROBLAST [J].