Resolution enhancement in hydrophobic interaction chromatography via electrostatic interactions

被引：6
|
作者：
Gao, Dong [1 ]
Tan, Fu-Chun [1 ]
Wang, Wen-Peng [1 ]
Wang, Li-Li [1 ]
机构：
[1] NW Univ Xian, Inst Modem Separat Sci, Shaanxi Key Lab Modem Separat Sci, Key Lab Synthet & Nat Funct Mol Chem,Minist Educ, Xian 710069, Peoples R China
来源：
CHINESE CHEMICAL LETTERS | 2013年 / 24卷 / 05期
关键词：
Hydrophobic interaction chromatography; Electrostatic interaction; Resolution; Hen egg white; HEN EGG-WHITE; PROTEINS; MACROMOLECULES; SEPARATION;
D O I：
10.1016/j.cclet.2013.03.004
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In this work, a new type of hydrophobic stationary phase that provide electrostatic interactions with analytes was developed by bonding beta-phenylethylamine as a functional ligand to silica. This stationary phase can separate proteins with similar hydrophobicity that traditional hydrophobic resins cannot. Hen egg white was separated to examine the selectivity. The results show that the introduced electrostatic interactions are an important factor for the resolution enhancement and the new resin could have important applications in separation and purification of biological macromolecules. (C) 2013 Doug Gao. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
引用
收藏
页码：419 / 421
页数：3
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